| 刘晓斌,侯明山,李 民,孟发财,黄卫东,宋锦宁.tBHQ和SFN在创伤性脑损伤小鼠中的疗效比较性分析[J].现代生物医学进展英文版,2017,17(28):5445-5448. |
| tBHQ和SFN在创伤性脑损伤小鼠中的疗效比较性分析 |
| Comparative Analysis of Therapeutic Effect between tBHQ and SFN in Rats with Traumatic Brain Injury |
| Received:December 06, 2016 Revised:December 30, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.28.010 |
| 中文关键词: 叔丁基对苯二酚 莱菔硫烷 脑损伤 Nrf2 NOX2 |
| 英文关键词: tBHQ SFN Traumatic brain injury Nrf2 NOX2 |
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| 中文摘要: |
| 摘要 目的：探讨叔丁基对苯二酚（tBHQ）和莱菔硫烷（SFN）在患有创伤性脑损伤大鼠的疗效差异性。方法：80只健康成年的雄性SD大鼠分为假手术组、常规损伤组、tBHQ治疗组和SFN治疗组，使用电子颅脑损伤仪（eCCI）制备TBI模型。其中tBHQ治疗组在伤前24 h大鼠腹腔注射三次tBHQ（50 mg/kg），每8 h一次；SFN治疗组在伤后15 min给予腹腔注射SFN（5 mg/kg）。给药24 h后，采用mNSS方法评价各组大鼠神经功能缺损状况，利用干湿称量法计算脑含水量，Western blot和ELISA方法分别测定大鼠脑组织的NOX2和Nrf2的表达水平。结果：损伤发生后第24 h，tBHQ治疗组和SFN治疗组在mNSS评分（(4.5±0.71) vs (9.2±0.79), (6.0±0.82) vs (9.2±0.79)）、脑水肿（79.4% vs 85.6%, 80.3% vs 85.6%）、NOX2和Nrf2（0.93 ng/mL vs 0.81 ng/mL, 0.87 ng/mL vs 0.81 ng/mL）表达上与常规损伤组差异明显，而tBHQ治疗组和SFN治疗组间在mNSS评分（(4.5±0.71) vs (6.0±0.82)）、NOX2和Nrf2（0.93 ng/mL vs 0.87 ng/mL）表达上差异显著。结论：在大鼠TBI模型中，tBHQ和SFN均可以有效的降低机体自身的氧化应激作用，并改善神经功能，但tBHQ的疗效要好于SFN。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the difference of therapeutic effect between tert-butylhydroquinone (tBHQ) and sulforaphane (SFN) in rats with traumatic brain injury. Methods: A total of 80 healthy adult male rats were randomly divided into 4 groups: sham group, TBI group, tBHQ treatment group and SFN treatment group, then the traumatic brain injury (TBI) model were prepared by eCCI. tBHQ treatment group rats were given intraperitoneal injection three times tBHQ (50 mg/kg) before injury 24 h, and SFN treatment group rats were given intraperitoneally injected SFN (5 mg/kg) after injury 15 min. Neurologic deficits was evaluated 24 h after TBI by mNSS method, brain edema was detected by the wet-dry ratio method. The expression levels of NOX2 protein and Nrf2 protein in the brain tissue were investigated using Western blot and ELISA analysised, respectively. Results: Compared with TBI group, there were significant difference on mNSS scores ((4.5±0.71) vs (9.2±0.79), (6.0±0.82) vs (9.2±0.79)), brain edema (79.4% vs 85.6%, 80.3% vs 85.6%), the expression levels of NOX2 protein and Nrf2 protein (0.93 ng/mL vs 0.81 ng/mL, 0.87 ng/mL vs 0.81 ng/mL) in tBHQ treatment group and SFN treatment group. In addition, there were also significant differences on mNSS scores ((4.5±0.71) vs (6.0±0.82)), the expression levels of NOX2 protein and Nrf2 protein (0.93 ng/mL vs 0.87 ng/mL) between tBHQ treatment group and SFN treatment group. Conclusion: In the TBI model of rats, tBHQ and SFN could effectively attenuated cerebral oxidative stress and improved nerve function after TBI, but the curative effect of tBHQ was better than the SFN. |
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