| 张 阳,周康熙,李 俊,耿英华,张 凤,杨艳丽.青蒿琥酯抑制白血病耐药细胞株K562/ADM转铁蛋白受体表达[J].现代生物医学进展英文版,2017,17(27):5233-5237. |
| 青蒿琥酯抑制白血病耐药细胞株K562/ADM转铁蛋白受体表达 |
| Effect of Artesunate on Transferrin Receptor in K562/ADM Cells |
| Received:March 19, 2017 Revised:April 15, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.27.008 |
| 中文关键词: 青蒿琥酯 转铁蛋白受体 K562/ADM细胞 |
| 英文关键词: Artesunate Transferrin receptor K562/ADM cells |
| 基金项目:蚌埠医学院2015年度研究生科研创新计划(Byycx1512) |
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| 中文摘要: |
| 摘要 目的：观察青蒿琥酯对于白血病多药耐药细胞细胞株K562/ADM转铁蛋白受体表达的影响。方法：将K562/ADM（耐阿霉素）细胞分别经浓度为12.5、25、50 μg/mL的青蒿琥酯处理48 h，同时25 μg/mL实验组在12 h、24 h、36 h分别收集足量细胞。采用流式细胞术检测青蒿琥酯对细胞转铁蛋白受体（TfR）密度的调控作用，Western blot检测青蒿琥酯对细胞TfR蛋白表达的调控作用。CCK-8法分析青蒿琥酯对K562/ADM细胞生长增殖的影响。结果：K562/ADM细胞TfR密度和TfR蛋白表达水平分别经12.5、25、50 μg/mL 青蒿琥酯处理后均下降，呈浓度依赖性。25 μg/mL青蒿琥酯处理K562/ADM细胞不同时间段后转铁蛋白受体蛋白表达水平随着Art作用时间延长而逐渐降低，表明呈时间依赖性。K562/ADM细胞经青蒿琥酯处理后其耐药性减弱：与对照组相比，12.5、25、50 μg/mL实验组细胞耐药逆转倍数分别为1.38、2.12和2.95倍，从而抑制K562/ADM细胞增殖。IC50值为19.7 μmol/L。结论：青蒿琥酯能降低细胞TfR密度和下调TfR蛋白的表达，逆转K562/ADM细胞的耐药性，从而起到抗肿瘤作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of Artesunate(Art) on the expression of transferrin receptor(TfR)in K562/ADM cells. Methods: The drug-resistant K562/ADM cells were cultured with 1000 ng/mL doxorubicin for two weeks followed by Artesunate treatment with different concentrations (12.5 μg/mL, 25μg/mL and 50 μg/mL) or different time (12 h, 24 h, 36 h, and 48 h). The content of transferrin receptor in K562/ADM cells was determined by flow cytometry. The effect of Artesunate on the expression of transferrin receptor protein in K562/ADM cells was measured by Western blot. Cell counting kit-8(CCK-8) assay was used to evaluate inhibitory effect of Art combined with doxorubicin (ADM) in K562/ADM cells. The reversal index was defined as the IC50 of the experimental group divided by the IC50 of the control group in K562/ADM cells. Results: Art effectively decreased the content of transferrin receptor and the expression of transferrin receptor protein in K562/ADM cells in a dose-dependent manner. Moreover, Art also inhibited transferrin receptor protein expression in K562/ADM cells in a time-dependent manner. The different concentrations of Art(12.5 μg/mL, 25μg/mL and 50 μg/mL) could induce reversal of drug-resistance with the reversal index being 1.38, 2.12 and 2.95 times (P<0.05). Art inhibited cell proliferation of K562/ADM cells, and the IC50 were19.7 μmol/L. Conclusion: Art effectively down-regulated the transferrin receptor content as well as transferrin receptor protein expression in K562/ADM cells, which resulted in reversal of drug resistance of K562/ADM cells. Art also inhibited K562/ADM cells proliferation, which has great value in clinical treatment of leukemia. |
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