| 曹丽华,殷丹丹,夏成才,王 宁,德 伟.lncRNA TUG1在胰岛β细胞分泌胰岛素中的功能研究[J].现代生物医学进展英文版,2017,17(25):4847-4851. |
| lncRNA TUG1在胰岛β细胞分泌胰岛素中的功能研究 |
| Function of lncRNA TUG1 in Insulin Secretion from Pancreatic Beta Cells |
| Received:November 18, 2016 Revised:December 15, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.25.011 |
| 中文关键词: LncRNA TUG1 β细胞 胰岛素分泌 |
| 英文关键词: LncRNA TUG1 β cells Insulin secretion |
| 基金项目:江苏省南京市医学科技发展资金项目（YKK13205）；江苏省南京市浦口医学科技发展资金项目（S2016-13） |
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| 中文摘要: |
| 摘要 目的：关于lncRNA TUG1在体内外胰岛?茁细胞分泌胰岛素中的功能研究。方法：通过qRT-PCR 检测lncRNA TUG1在小鼠胰腺，脑，肌肉等不同组织的表达。体外干扰MIN6胰岛素瘤细胞系lncRNA TUG1后，通过MTT法和流式细胞计数检测对β细胞增殖和周期影响；通过GSIS检测β细胞不同糖浓度刺激下的胰岛素分泌水平；采用qRT-PCR 检测β细胞Insulin及相关特异转录因子Pdx1, MafA, NeuroD, Glut2的变化；外源性封闭正常成年小鼠中lncRNA TUG1的表达后，采用ELISA法检测对血清胰岛素的影响，采用免疫组化检测对胰岛形态的影响。结果：lncRNA TUG1在胰腺组织中高度表达。干扰lncRNA TUG1后可致β细胞增殖活力受到抑制，糖刺激下的胰岛素分泌水平下降，Insulin及相关特异转录因子Pdx1, MafA, NeuroD,Glut2减少；外源性封闭正常成年小鼠中lncRNA TUG1的表达后，血清胰岛素减少，胰岛面积减小。结论：干扰lncRNA TUG1后在体内外均可导致胰腺β细胞分泌胰岛素减少，提示lncRNA TUG1可在体内外影响?茁细胞的胰岛素分泌，lncRNA TUG1是调节胰岛?茁细胞功能的因素之一。 |
| 英文摘要: |
| ABSTRACT Objective: To determine the role of lncRNA TUG1 in pancreatic β cells functioning both in vitro and in vivo. Methods: The lncRNA TUG1 expression in mice pancreas, brain, muscle and other different tissues was examined through qRT - PCR. MTT, flow cytometry, GSIS, ELISA and immunochemistry analyses were performed to detect the effect of lncRNA TUG1 on insulin secretion in vit- ro and in vivo. Results: lncRNA TUG1 was highly expressed in pancreatic tissue compared with other organ tissues. Knockdown of lncRNA TUG1 expression resulted in decreased insulin secretion in β cells both in vitro and in vivo. Immunochemistry analyses showed decreased relative islet area after treatment with lncRNA TUG1 siRNA. Conclusion: Downregulation of lncRNA TUG1 expression can affect insulin secretion in pancreatic β cells in vitro and in vivo, and lncRNA TUG1 may represent a factor that regulates the function of pancreatic β cells. |
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