石 嵩,栾 晓,柳 洋,郭菲菲,徐 珞.大鼠下丘脑LHA-PVN nesfatin-1神经通路构成及对胃运动的影响[J].现代生物医学进展英文版,2017,17(25):4831-4836. |
大鼠下丘脑LHA-PVN nesfatin-1神经通路构成及对胃运动的影响 |
The Effects of Nesfatin-1 in the Paraventricular Nucleus on Gastric Motility and Its Potential Regulation by the Lateral Hypothalamic Area in Rats |
Received:March 03, 2017 Revised:March 28, 2017 |
DOI:10.13241/j.cnki.pmb.2017.25.008 |
中文关键词: 胃牵张敏感神经元 胃运动 下丘脑外侧核 nesfatin-1 下丘脑室旁核 |
英文关键词: Gastric distension responsive neurons Gastric motility Lateral hypothalamic area Nesfatin-1 Paraventricular nucleus |
基金项目:国家自然科学基金项目(81470815,81270460,81500414);山东省优秀中青年科学基金项目(BS2014YY009);青岛市科技局项目(14-2-3-3-nsh) |
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中文摘要: |
摘要 目的:阐述LHA和PVN间nesfatin-1神经通路的构成,探讨PVN nesfatin-1对胃收缩幅度及频率的影响及潜在机制。方法:采用荧光金逆行追踪结合荧光免疫组化技术,观察LHA-PVN间nesfatin-1神经通路构成;采用细胞外放电记录,观察nesfatin-1对GD放点活动的影响;通过在体胃运动技术,观察nesfatin-1对清醒自由活动大鼠胃收缩幅度和频率的影响。结果:Nesfatin-1能够抑制GD-E神经元放电(1.97±0.12 Hz vs. 1.15±0.07 Hz)促进GD-I神经元放电(1.74±0.10 Hz vs. 3.04±0.18 Hz),H4928能够部分阻断nesfatin-1对GD神经元(GD-E:1.38±0.08 Hz,P<0.05 vs. nesfatin-1; GD-I:2.49±0.15 Hz,P<0.05 vs. nesfatin-1)的影响;PVN内微量注射nesfatin-1能够抑制大鼠胃运动,呈量效依赖关系;LHA和PVN间有nesfatin-1神经纤维联系;电刺激LHA后,GD神经元放电频率增加(GD-E:2.06±0.12 Hz vs. 4.23±0.21 Hz,GD-I:1.61±0.09 Hz vs. 4.83±0.25 Hz),预先向PVN注射抗NUCB2/nesfatin-1抗体后,GD-E神经元放电频率减弱(4.91±0.25 Hz vs. 4.23±0.21 Hz),而GD-I神经元放电频率增强(4.15±0.18 Hz vs. 4.83±0.25)。结论:PVN内nesfatin-1可调控大鼠GD神经元放电活动及胃运动,该效应受LHA调控。 |
英文摘要: |
ABSTRACT Objective: The current study investigated the effects of nesfatin-1 in the hypothalamic paraventricular nucleus (PVN) on gastric motility and the regulation of the lateral hypothalamic area (LHA). Methods: The projection of nerve ?ber and expression of nesfatin-1 were observed by retrograde tracing and fuo-immunohistochemistry staining; The nuclei microinjection and nuclei electrical stimulation, extracellular discharges of single unit neuron were used to observe the effects of nesfatin-1 on the GD neurons; Gastric motil- ity recording in vivo were used to monitor the effects of nesfatin-1 on the amplitude of constriction and frequency of gastric motility in conscious rats. Results: Nesfatin-1 inhibited the majority of the GD-E neurons(1.97±0.12 Hz vs. 1.15±0.07 Hz) and excited GD-I neu- rons(1.74±0.10 Hz vs. 3.04±0.18 Hz) in the PVN, which were weakened by oxytocin receptor antagonist H4928(GD-E: 1.38±0.08 Hz, P<0.05 vs. nesfatin-1; GD-I: 2.49±0.15 Hz, P<0.05 vs. nesfatin-1). Gastric motility experiments showed that administration of nes- fatin-1 in the PVN decreased gastric motility. Retrograde tracing and immunofuorescent staining showed that nucleobindin-2/nesfatin-1 and fuorogold double-labeled neurons were observed in the LHA. Electrical LHA stimulation excited the firing rate of GD-responsive neurons(GD-E: 2.06±0.12 Hz vs. 4.23±0.21 Hz, GD-I: 1.61±0.09 Hz vs. 4.83±0.25 Hz) in the PVN. Pre-administration of an anti- nucleobindin-2/nesfatin-1 antibody in the PVN strengthened gastric motility, decreased GD-E neurons(1.74±0.10 Hz vs. 3.04±0.18 Hz) and excited the discharging of the GD-I neurons(4.15±0.18 Hz vs. 4.83±0.25) induced by electrical stimulation of the LHA. Conclusion: Nesfatin-1 in the PVN could serve as an inhibitory factor to inhibit gastric motility, which might be regulated by the LHA. |
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