| 罗年安,陈玉宝,武晓军,董 瑞.乳腺癌细胞中HuR调控PDFC的分子机制研究[J].现代生物医学进展英文版,2017,17(25):4828-4830. |
| 乳腺癌细胞中HuR调控PDFC的分子机制研究 |
| Molecular Mechanism of PDGFC Regulated by HuR in Breast Cancer Cells |
| Received:December 09, 2016 Revised:December 29, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.25.007 |
| 中文关键词: 乳腺肿瘤 HuR PDGFC 分子机制 |
| 英文关键词: Breast neoplasms HuR PDGFC Molecular mechanism |
| 基金项目:国家自然科学基金项目（81001181）；陕西省自然科学基金项目（2016JM8066） |
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| 中文摘要: |
| 摘要 目的：探讨乳腺癌细胞中HuR调控PDGFC的分子机制。方法：通过软件预测分析，乳腺癌细胞中PDGFC 3'UTR的HuR结合位点；在RNA免疫共沉淀实验中，加入PDGFC刺激后检测HuR与PDGFC mRNA的相互作用；通过构建PDGFC 3'UTR五个截短体，荧光素酶报告基因实验检测HuR调控PDGFC的结合位点。结果：软件预测分析发现，PDGFC 3'UTR可能存在五个HuR结合位点；RNA免疫共沉淀实验中，当加入PDGFC刺激后，HuR与PDGFC mRNA出现免疫共沉淀，证明HuR和PDGFC之间的直接相互作用；PDGFC mRNA3'UTR报告基因系统检测显示，第2个和第4个位点可与HuR结合调控 PDGFC。结论：本研究揭示了乳腺癌细胞中HuR通过与PDGFC mRNA 3'UTR结合调控PDGFC的分子机制，为乳腺癌的临床诊断和治疗提供了新的思路。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the molecular mechanism of PDGFC regulated by HuR in breast cancer cells. Methods: Through the software analysises, we first predicted the HuR-binding sites on the PDGFC 3'-UTR in breast cancer cells; An RNA-im- munoprecipitation tested the interaction of HuR with the PDGFC mRN after adding PDGFC stimulation; Luciferase experiments tested the HuR-binding sites of PDGFC regulated by HuR through structuring five truncated of PDGFC 3'UTR. Results: We found five HuR-binding sites in the 3'UTR of PDGFC by software prediction; The RNA-immunoprecipitation showed the co-immunoprecipitation of HuR and PDGFC mRN after adding PDGFC stimulation confirming the direct association of HuR with the PDGFC; Luciferase experi- ments of PDGFC mRNA 3'UTR showed that PDGFC regulated by the second and fourth HuR-binding sites. Conclusion: This study re- veals the molecular mechanism of PDGFC regulated by HuR through binding to PDGFC mRNA 3'UTR in breast cancer cells, and pro- vided rationale for the development of strategies in the clinical diagnosis and treatment for breast cancer. |
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