| 雍 军,李林格,李 亮,马遇庆,冯 娟,范宇䔷,古扎力努尔,尼力帕尔,王 松,张 华.鼻息肉中HIF-1α、VEGF 和miR-200a 表达及其与复发相关性研究[J].现代生物医学进展英文版,2017,17(21):4059-4062. |
| 鼻息肉中HIF-1α、VEGF 和miR-200a 表达及其与复发相关性研究 |
| Expressions of HIF-1α, VEGF and miR-200a and Its Correlation with Recurrence of Nasal Polyps |
| Received:November 30, 2016 Revised:December 23, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.21.014 |
| 中文关键词: 鼻息肉 血管内皮生长因子 缺氧诱导因子-1α miR-200a |
| 英文关键词: Nasal polyp VEGF HIF-1α miR-200a |
| 基金项目:国家自然科学基金项目（81460094） |
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| 中文摘要: |
| 摘要 目的：分析不同病理分型鼻息肉中HIF-1α、VEGF 和miR-200a 表达及其与复发相关性研究。方法：选取鼻息肉患者42例，在随访期间有15例鼻息肉复发。采用免疫组化SABC法检测鼻息肉及下鼻甲组织中HIF-1α、VEGF表达水平，采用qRT-PCR技术检测鼻息肉及下鼻甲粘膜组织中miRNA-200a表达量。对比分析不同病理分型HIF-1α、VEGF、miRNA-200a表达差异并分析鼻息肉复发的因素。结果：鼻息肉中miR-200a表达量明显低于下鼻甲粘膜组织(P<0.05)。鼻息肉中HIF-1α、VEGF表达量明显高于于下鼻甲粘膜组织(P<0.05)。鼻息肉病组织中miR-200a表达量明显低于单发鼻息肉、多发鼻息肉(P<0.05)；鼻息肉病标本中HIF-1α、VEGF表达量明显高于单发鼻息肉、多发鼻息肉组织(P<0.05)。鼻息肉复发与鼻息肉的病理分型、HIF-1α、VEGF表达密切相关(P<0.05)。结论：鼻息肉增生和局部血管的生成密切相关，HIF-1α可能是同构调节miR-200a表达，来控制VEGF及血管生成的。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the expressions of HIF-1α, VEGF and miR-200a in different pathological types of nasal polyps and its correlation with recurrence. Methods: The data of 42 patients with nasal polyps were selected, and 15 of them had recurrence dur- ing the follow-up period. The immunohistochemical SABC method was applied to detect expression levels of HIF-1α and VEGF in nasal polyps and inferior turbinate tissues. The qRT-PCR technique was used to detect the microrRNA 200a quantity expression in nasal polyps and inferior turbinate mucosa tissues. Compare the differences of expressions of HIF-1α, VEGF and miRNA-200a in different pathological classifications and analyze the factors of recurrence of nasal polyps. Results: The miR-200a expression in nasal polyps was significantly lower than in the inferior turbinate mucosa tissue (P<0.05). The HIF-1α and VEGF expressions in nasal polyps were signifi- cantly higher than in inferior turbinate mucosa tissues (P<0.05). The miR-200a expression level in nasal polyposis tissues was obviously lower than in tissues of single nasal polyps and multiple nasal polyps (P<0.05). The expressions of HIF-1α and VEGF in nasal polyposis specimens were obviously higher than in tissues of single nasal polyps and multiple nasal polyps (P<0.05). The recurrence of nasal polyps was closely related to the nasal polyps pathological classifications and expressions of HIF-1α and VEGF (P<0.05). Conclusion: Nasal polyps hyperplasia had close relation with the local blood vessel formation. HIF-1α may control the VEGF and angiogenesis by homo- geneously regulating the miR-200a expression. |
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