| 易 星,刘兴发,石琳钰,邹 游,戴梦源,陶泽璋,肖伯奎,张建功,陈始明.变压器噪声暴露对SD大鼠听力及应激状态的影响研究[J].现代生物医学进展英文版,2017,17(20):3836-3841. |
| 变压器噪声暴露对SD大鼠听力及应激状态的影响研究 |
| Effects of Transformer Noise on Auditory Function and Stress State in SD Rats |
| Received:February 27, 2017 Revised:March 19, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.20.008 |
| 中文关键词: 变压器噪声 SD大鼠 听力学 耳蜗铺片与听毛细胞计数 应激 |
| 英文关键词: Transformer noise SD rats Auditory function Cochlear hair cells preparation and count Stress |
| 基金项目:国家电网公司总部科技项目（GY71-13-057） |
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| 中文摘要: |
| 摘要 目的：探究短时间内低声级强度低频的变压器噪声暴露对SD大鼠听力及应激状态方面的影响。方法：选取90只SPF级健康无听力障碍的（雌雄各半）SD大鼠作为实验对象，随机分为实验A、B组和对照C组，A、B组分别给予声级上限为65 dB SPL、60 dB SPL（频谱范围：100~800 Hz）的变压器噪声，噪声暴露时程为8周，每日噪声给予时间为22点至次日8点，C组在相同条件下饲养，不给予噪声暴露。噪声暴露结束后，通过DPOAE（畸变耳声发射）、ABR（听性脑干反应）检测、耳蜗铺片及毛细胞计数对SD大鼠听力学状况进行评估；通过血清中促肾上腺皮质激素（ACTH）、血清皮质醇（CORT）对SD大鼠的应激状态进行评估。结果：在变压器噪声暴露的8周内，各组大鼠生长状况良好，体重均呈正常生理性增长，组间无明显差异(P>0.05)；在变压器噪声暴露8周后，对A、B、C三组大鼠的听力学指标进行两两比较，组间均无明显差异(P>0.05)，对大鼠血清中促肾上腺皮质激素(ACTH)、血清皮质醇(CORT)的含量进行三组间比较，组间差异均无统计学意义(P>0.05)。结论：连续暴露于声压级上限65/60 dB SPL，频谱范围为100~800 Hz的变压器噪声下8周（10小时/天）对SD大鼠听力未产生明显影响，未引发SD大鼠应激状态。 |
| 英文摘要: |
| ABSTRACT Objective: To determine the effect of short-term transformer noise on auditory function and stress state in SD rats. Methods: 90 healthy SD rats (half male and female) with normal hearing were randomly divided into experimental group A, B and control group C. The experimental groups were exposed to the recorded transformer noise for 8 weeks (10 hours per day, 10:00 pm~8:00 am). Group A was exposed to the pressure level 65 dB SPL at Max (noise spectrum: 100-800 Hz), and group B was exposed to the pressure level 60 dB SPL at Max (noise spectrum: 100-800 Hz), control group C were maintained under the same conditions, without noise stimulation. The indexes of auditory function and stress state of SD rats were determined and compared among the three groups after an 8 weeks' noise exposure. Results: In the 8 weeks of transformer noise exposure, the rats showed normal physiological growth, and there was no significant difference (P>0.05) among group A, B and C. After noise exposure, indices of the auditory function (distortion product otoacoustic emission, DPOAE; auditory brainstem response, ABR) and the counting and observation of cochlear hair cells of the groups were compared, and no statistically significant difference was observed (P> 0.05). In addition, there were no significant differences in the ACTH and CORT content changes among the serums of three groups (P>0.05). Conclusion: Continuous exposure to transformer noise (sound pressure level 60/65 dB SPL at Max, noise spectrum: 100~800 Hz) for 8 weeks (10 hours/day) had no significant effect on the auditory function of SD rats, and never started the stress state in rats. |
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