| 刘 磊,胡春杰,李志杰,安 然,赵宏辉.水飞蓟宾诱导卵巢癌HO-8910细胞凋亡增殖及其机制探讨[J].现代生物医学进展英文版,2017,17(20):3811-3815. |
| 水飞蓟宾诱导卵巢癌HO-8910细胞凋亡增殖及其机制探讨 |
| Silibinin Inhibite the Survival of Ovarian Cancer HO-8910 Cells and Its Mechanism |
| Received:October 27, 2016 Revised:November 20, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.20.003 |
| 中文关键词: 水飞蓟宾 HO-8910细胞 凋亡 bax bcl-2 |
| 英文关键词: Silibinin HO-8910 cells Apoptosis Bax Bcl-2 |
| 基金项目:黑龙江省自然科学基金项目（H201424） |
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| 中文摘要: |
| 摘要 目的：探讨水飞蓟宾对人卵巢癌 HO-8910细胞增殖的抑制作用及其作用机制。方法：HO-8910细胞分为四组：(1)对照组；(2) 水飞蓟宾低浓度组；(3)水飞蓟宾中浓度组；(4)水飞蓟宾高浓度组。通过MTT法测定细胞增值率，流式细胞技术检测细胞凋亡情况，Hoechst染色观查细胞核凋亡，Western-blot检测bax及bcl-2表达。结果：细胞增殖检测结果显示，水飞蓟宾低、中、高浓度组的抑制率（83.00±5.51%、65.33±3.48%、56.67±4.37%）与对照组（97.33±4.25%）比较差异具有统计学意义（P <0.05）。流式细胞技术结果显示，水飞蓟宾低、中、高浓度组凋亡细胞比例分别为16.93±2.34%、26.20±2.21%和37.93±1.98%，与对照组（1.43±0.72%）相比差异均有统计学意义 (P<0.05)。Hoechst染色结果显示，水飞蓟宾低、中、高浓度组凋亡细胞比例分别为12.56±2.55%、25.73±2.05%和39.14±3.69%，与对照组（0.54±0.67%）相比差异均有统计学意义 (P<0.05)。此外，水飞蓟宾可以升高bax基因表达水平，降低bcl-2基因表达平。结论：水飞蓟宾能明显抑制HO-8910细胞增殖，促进细胞凋亡，通过改变凋亡因子表达诱导卵巢癌HO-8910细胞凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the inhibit effect and mechanism of silibinin on human ovarian cancer HO-8910 cells' proliferation. Methods: HO-8910 cells were divided into four groups: (1) negative control group; (2) silibinin low dose group; (3) silibinin middle dose group; (4) silibinin high dose group. Cell proliferation rate of each group of HO-8910 cells were test by MTT assay, cells apoptosis rate were tested by flow cytometry assay, nuclei apoptosis were checked by hoechst staine, bax and bcl-2 expressions were detected by Western-blot. Results: Cell survival test results showed that inhibition rate of silibinin low, middle and high dose group was (83.00±5.51%, 65.33±3.48%, 56.67±4.37%) respectively, which have statistics meaning(P<0.05) when compared with control group(97.33±4.25%); Flow cytometry results showed that the percentage of apoptosis cells of silibinin low, middle, high dose group was 12.56±2.55%, 25.73±2.05% and 39.14±3.69% respectively, which have statistics meaning(P<0.05) when compared with control group(1.43±0.72%); Hoechst results showed that percentage of apoptosis cells of silibinin low, middle, high dose group was 12.56±2.55%, 25.73±2.05% and 39.14±3.69% respectively, which have statistics meaning(P<0.05) when compared with control group(0.54±0.67%); Besides, silibinin could increase bax gene expression levels and decrease bcl-2 gene expression levels. Conclusion: Silibinin could effective inhibit HO-8910 cell proliferation and promote apoptosis, Silibinin induced ovarian cancer HO-8910 cells apoptosis by changing the expression of apoptosis genes. |
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