| 田 爱,夏志强,黄勇亮,李万狮,许永春.唑来膦酸联合IL-2体外诱导胃癌患者外周血γδT细胞的研究[J].现代生物医学进展英文版,2017,17(19):3750-3753. |
| 唑来膦酸联合IL-2体外诱导胃癌患者外周血γδT细胞的研究 |
| Effects of Zoledronate and Interlukin 2 on the Proliferation of Peripheral Blood Mononuclear Cells of Patients with Gastric Cancer in Vitro |
| Received:January 18, 2017 Revised:February 22, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.19.038 |
| 中文关键词: γδT细胞 胃癌 唑来膦酸 IL-2 过继性免疫治疗 |
| 英文关键词: γδT cells Gastic cancer Zeledronate IL-2 Adoptive immunotherapy |
| 基金项目:国家自然科学基金项目（81600451） |
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| 中文摘要: |
| 摘要 目的：探讨唑来膦酸联合白细胞介素-2(IL-2)诱导胃癌患者外周血γδT细胞的作用。方法：收集胃癌患者和健康捐献者各30例，分别采集外周静脉血并分离出单核细胞(PBMC)，采用流式细胞仪(FCM)检测唑来膦酸联合IL-2诱导前后γδT细胞的数量及其CD69分子的表达情况。结果：胃癌组的γδT细胞数量和百分比均明显低于健康对照组(P<0.05)；唑来膦酸联合IL-2诱导PBMC后，胃癌实验组和健康对照组中的γδT细胞的数量和百分比均较诱导前明显升高(P<0.05)。结论：唑来膦酸联合IL-2体外诱导PBMC可显著扩增γδT细胞，以加强杀伤肿瘤细胞的目的。 |
| 英文摘要: |
| ABSTRACT Objective: To determine the effects of zoledronate plus interlukin 2(IL-2) on the proliferation of peripheral blood mononuclear cells (PBMCs) of patients with gastric cancer in vitro. Methods: PBMCs were isolated from 30 healthy doners and 30 pa- tients with gastric cancer respectively, and then stimulated with zoledronate plus IL-2 in vitro. Flow cytometry(FCM) was used to evalu- ate the γδT cell subpopulation of PBMCs and detect the expression of CD69 in γδT cells. Results: The percentage and absolute number of γδT cells from PBMCs were both lower in patients with gastric cancer than those of the control group (P<0.05). The combination of zoledronate and interlukin 2 could significantly increase the percentage and absolute number of γδT cells from PBMCs in patients with gastric cancer and healthy control group(P<0.05). Conclusion: Zoledronate and IL-2 could obviously stimulate the proliferation of γδT cells from PBMCs to further kill the cancer cells. |
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