| 范星星,卜淑敏,郭 盖,孔令华,陈冠洁.跑台运动和饮食控制对去卵巢肥胖大鼠心肌Wnt/β-catenin信号通路的影响[J].现代生物医学进展英文版,2017,17(19):3646-3650. |
| 跑台运动和饮食控制对去卵巢肥胖大鼠心肌Wnt/β-catenin信号通路的影响 |
| Effects of Treadmill Exercise and Feeding Control on the Wnt /β-catenin Signaling Pathway in the Myocardium of Ovariectomized Fat Rats |
| Received:December 11, 2016 Revised:December 29, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.19.011 |
| 中文关键词: 去卵巢大鼠 跑台运动 心肌 Wnt/β-catenin信号通路 |
| 英文关键词: Ovariectomized rats Treadmill exercise Myocardium Wnt/ β-catenin signal pathway |
| 基金项目:北京市百千万人才工程项目（201624） |
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| 中文摘要: |
| 摘要 目的：观察跑台运动和饮食控制对去卵巢肥胖大鼠心肌Wnt/β-catenin信号通路中β-catenin、磷酸化-GSK-3β和GSK-3β蛋白表达的影响。方法：将48只3月龄雌性未孕SD大鼠按体重分层后随机分为假手术组、去卵巢组、去卵巢运动组和去卵巢控食组。去卵巢运动组大鼠在去卵巢手术后的第3周开始进行为期8周的跑台运动训练，去卵巢控食组在去卵巢后的第三周开始进行为期8周的控食干预。干预结束后，腹主动脉取血处死各组大鼠，用电子天平秤量大鼠心肌重量，用Western blot方法检测心肌β-catenin、磷酸化-GSK-3β和GSK-3β蛋白表达。结果：与假手术组大鼠比较，去卵巢组大鼠体重、心肌重量显著增加，而心肌重量指数、β-catenin、磷酸化GSK-3β和p-GSK-3β/GSK-3β蛋白表达均显著降低(P<0.05)；与去卵巢组大鼠比较，去卵巢运动组大鼠在最后两周时体重显著降低(P<0.05)，心肌组织β-catenin、p-GSK-3β和p-GSK-3β/GSK-3β蛋白表达均显著升高，而去卵巢控食组大鼠体重、心肌重量、心肌重量指数和GSK-3β表达差异均无统计学意义(P>0.05)，但心肌组织β-catenin、p-GSK-3β和p-GSK-3β/GSK-3β蛋白表达均显著升高(P<0.05)。结论：跑台运动和控食干均预均能激活去卵巢大鼠心肌Wnt/β-catenin信号通路。 |
| 英文摘要: |
| ABSTRACT Objective: To observe the effects of treadmill exercise and feeding control on the protein expression of β-catenin and GSK-3β in the myocardium of ovariectomized fat rats. Methods: Forty-eight healthy 3-month-old female SD rats were randomly divided into four groups accordin to the body weight: sham-operated group, ovariectomized group, treadmill exercise group and feeding control group. At the three weeks after ovariectomy, the treadmill exercise group started treadmill exercise training and the feeding control group started feeding control. During the 24-48 hours of the final treatment, all the rats were sacrificed by abdominal aorta. The myocardial weights of rats were measured with an electronic balance. The protein expression of β-catenin, p- GSK-3β and GSK-3β were detected by Western blot. Results: Compared with sham-operated group, the weight body and myocardial mass of OVX group were significantly in- creased, while the myocardial mass/weight body, β-catenin, Phosphorylated GSK-3β and p-GSK-3β/GSK-3β were significantly de- creased (p<0.05); Compared with OVX group, the weight body of TE group were significantly increased in the last two weeks(P<0.05), but Myocardial tissue β-catenin, p-GSK-3β and p-GSK-3β/GSK-3β were significantly increased, whereas CF Group did not exhibit the difference of the weight body, myocardial mass, myocardial mass/weight body and GSK-3β expression. But Myocardial tissue β-catenin, p-GSK-3β and p-GSK-3β/GSK-3β were significantly increased(P<0.05). Conclusion: Both treadmill exercise and diet control could acti- vate the Wnt / β-catenin signal pathway in myo-cardium of ovariectomized fat rats. |
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