| 迟男男,苏德望,于佳楠,陈 颖,程 开.胆管上皮细胞TLR3内源性活化在原发性胆汁性肝硬化中的作用[J].现代生物医学进展英文版,2017,17(19):3625-3627. |
| 胆管上皮细胞TLR3内源性活化在原发性胆汁性肝硬化中的作用 |
| Role of Endogenous TLR3 Activation in Primary Biliary Cirrhosi |
| Received:August 20, 2016 Revised:September 20, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.19.006 |
| 中文关键词: Toll样受体3 胆管上皮细胞 原发性胆汁性肝硬化 内源性活化 |
| 英文关键词: Toll like receptor 3 Bile duct epithelial cells Primary biliary cirrhosis Endogenous activation |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：探讨胆管上皮细胞中Toll样受体3（TLR3）内源性活化对原发性胆汁性肝硬化损伤的影响。方法：体外培养人肝内胆管上皮细胞（HiBEC），并通过冻融处理制备坏死HiBEC样本。将死亡样本与活样本混合培养6 h，以制备TLR3内源性活化样本，将其标记为观察组；以核酸酶处理坏死细胞及活细胞，并混合培养6 h，以制备对照样本，将其标记为对照组。检测2组细胞凋亡率、TLR3及β干扰素Toll样受体结构域衔接蛋白（TRIF）表达水平、Caspase-3活性。结果：观察组凋亡率明显高于对照组，TLR3及TRIF相对表达量明显高于对照组，细胞Caspase-3平均表达量明显高于对照组，上述差异均有统计学意义（P＜0.05）。结论：TLR3的内源性活化可导致HiBEC凋亡，在原发性胆汁性肝硬化的发生及发展中可能有一定促进作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of Toll like receptor 3 (TLR3) on the injury of primary biliary cirrhosis in bile duct epithelial cells. Methods: The human intrahepatic bile duct epithelial cells (HiBEC) were cultured in vitro, and HiBEC samples were pre- pared by freezing and thawing treatment. Death samples and the live samples were mixed and cultured for 6h to prepare TLR3 endoge- nous activated samples, labeled as observation group. After treated with nuclease, the necrotic cells and live cells were mixed and cul- tured for 6h to prepare a control sample, labeled as control group. The apoptosis rate, the expression level of TLR3 and the expression level of β interferon-Toll-like receptor domain adaptor protein (TRIF) and the Caspase-3 activity were detected in the two groups. Results: The apoptosis rate of the observation group was significantly higher than that of the control group. The TLR3 and TRIF relative expres- sion of the observation group was also significantly higher than that of the control group. Moreover, the caspase-3 average expression of the observation group was obviously higher than that of the control group. All the above mentioned differences were statistically signifi- cant (P<0.05). Conclusion: The endogenous activation of TLR3 may lead to HiBEC apoptosis. It may promote the occurrence and devel- opment of primary biliary cirrhosis. |
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