| 张冬子,金 林,行治国,陈 斌,闫中南.表没食子儿茶素没食子酸酯对蛛网膜下腔出血大鼠脑损伤的保护作用研究[J].现代生物医学进展英文版,2017,17(19):3620-3624. |
| 表没食子儿茶素没食子酸酯对蛛网膜下腔出血大鼠脑损伤的保护作用研究 |
| Protective Effects of (-)-Epigallocatechin-3-Gallate on the Brain Injury Induced by Subarachnoid Hemorrhage in Rats |
| Received:January 10, 2017 Revised:February 04, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.19.005 |
| 中文关键词: 表没食子儿茶素没食子酸酯 蛛网膜下腔出血 脑损伤 神经功能 BDNF TrkB |
| 英文关键词: (-)-Epigallocatechin-3-Gallate Subarachnoid hemorrhage Brain injury Neurological function BDNF TrKB |
| 基金项目:陕西省教育厅专项科研计划项目（2013JK0787） |
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| 中文摘要: |
| 摘要 目的：探讨表没食子儿茶素没食子酸酯(EGCG)对蛛网膜下腔出血(SAH)脑损伤的影响及其可能机制。方法：选取健康雄性Sprague-Dawley(SD)大鼠80 只，按照随机数字表法分为假手术组（Sham组）、SAH组、SAH+EGCG组、蛛网膜下出血+生理盐水组（SAH+NS组），每组各20 只。SAH造模成功后1 d、3 d，检测大鼠神经功能评分及脑组织含水量；造模成功3 d后，脑组织石蜡切片HE染色观察脑细胞形态，并采用超敏C-反应蛋白(hsCRP)试剂盒检测血清hsCRP水平，通过Western blotting检测脑组织脑源性神经生长因子(brain derived neurotrophic factor，BDNF)及与其高度亲和的酪氨酸激酶受体B(tropomyosin-related kinase B，TrkB)的表达。结果：①SAH+EGCG组神经功能评分较Sham组明显降低，但较同期SAH组增高明显(P<0.05)；②与Sham组比较，SAH+EGCG组脑组织含水量明显增加，但与同期SAH组比较，SAH+EGCG组脑组织含水量明显降低(P<0.05)；③与SAH组比较，SAH+EGCG组血清hsCRP水平明显降低(P<0.05)；④与SAH组比较，SAH+EGCG组脑组织石蜡切片HE染色神经细胞水肿明显减轻，且坏死神经元减少，细胞核固缩明显减轻；⑤与SAH组比较，SAH+EGCG组脑组织BDNF、TrkB的表达量明显升高(P<0.05)。结论：EGCG能够有效的保护大鼠SAH后的脑损伤，促进神经功能修复，减轻预后神经功能障碍，可能与其促进脑组织BDNF、TrkB的表达有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of EGCG on the brain injury induced by subarachnoid hemorrhage (SAH) and the possible mechanisms. Methods: 80 healthy SD male rats were randomly divided into the Sham group (n=20), SAH group (n=20), SAH+EGCG group (n=20), SAH+NS group (n=20). Then the neurological function score and brain water content after the success of the SAH model on the 1 st , 3 rd day were measured and the brain cells morphology was observed by HE staining on the 3 rd day, the levels of serum hsCRP by hsCRP kit were and detected on the 3 rd day, and the expression of BDNF and TrkB in brain tissue were detected by Western blotting on the 3 rd day. Results: ①Compared with the Sham group, the neurological function score of SAH+EGCG group was lower, however, the neurological function score of SAH+EGCG group was higher than the SAH group at the same period (P<0.05); ②Compared with the Sham group, the brain water content of SAH+EGCG group was significantly increased, but compared with the SAH group, the brain water content of SAH EGCG group was significantly lower(P<0.05). ③Compared with the SAH group, the serum hsCRP level in the SAH+EGCG group was significantly lower(P<0.05). ④Compared with the SAH group, the edema of nerve cells was decreased in the brain tissue of SAH+EGCG group, the necrotic neurons were reduced and the nuclear pyknosis was significantly reduced. ⑤Compared with the SAH group, the expressions of BDNF and TrkB in the brain tissue were significantly increased(P<0.05). Conclusion: EGCG could effectively protect the brain injury induced by SAH and promote the recovery of nerve function and reduce the prognosis, which might be related to increase the expressions of BDNF and TrKB in the brain tissue. |
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