| 戚 瑞,王 颖,王晓慧,张 磊,徐孝娜,谢祥军,杨瑞华.维生素D对高脂诱导的2型糖尿病大鼠海马神经元损伤的影响[J].现代生物医学进展英文版,2017,17(19):3615-3619. |
| 维生素D对高脂诱导的2型糖尿病大鼠海马神经元损伤的影响 |
| The Effect of Vitamin D on the Hippocampal Neurons in Type 2 Diabetic Rats Induced by High-fat Diet |
| Received:February 11, 2017 Revised:March 06, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.19.004 |
| 中文关键词: 高脂膳食 2型糖尿病 维生素D 认知障碍 海马神经元 |
| 英文关键词: High-fat diet Type 2 diabetes Vitamin D Organization disorder Hippocampal neuron |
| 基金项目:国家自然科学基金面上项目(81271194)；陕西省自然科学基金项目(2011JM4002) |
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| 中文摘要: |
| 摘要 目的：观察活性维生素 D3 腹腔注射对2型糖尿病大鼠海马神经元的影响。方法：SD大鼠30只，随机分为空白对照组（Control），高脂组（HF），高脂+VD组（HF+VD），糖尿病组(DM)，糖尿病+VD组(DM+VD)。测定大鼠空腹血糖、胰岛素、维生素D等生化指标，应用透射电镜观察海马神经元结构变化。结果：与对照组相比，HF组、HF+VD组大鼠体重增加，（分别为343.28±10.41 g；356.81±36.20 g和360.18±25.56g）， DM组体重降低(265.6±16.11 g)（P<0.05）；DM组SDF-1为398.33±15.01 ng/L，低于Control组(457.53±26.56 ng/L)，骨化三醇干预后SDF-1显著升高（448.54±36.83 ng/L）（P<0.05）；DM组（25-OH-D）水平为165.25±30.75 μg/L，低于Control组213.65±30.79 μg/L，补充骨化三醇后显著提高至210.31±50.69 μg/L（P<0.05）；DM组海马神经元CXCR4表达降低，线粒体可见明显肿胀，DM+VD组线粒体形态明显改善，海马神经元CXCR4表达水平明显升高。结论：常规量补充维生素D可提高糖尿病大鼠体内维生素D水平，对海马神经元有明显保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effect of vitamin D3 on the hippocampal neurons of type 2 diabetic rats. Methods: 30 female SD rats were randomly divided into control group, high fat group (HF), high fat + vitamin D (HF + VD), diabetes mellitus group (DM), diabetes mellitus + vitamin D (DM + VD). The blood sugar, insulin and vitamin D level were detected at the beginning and end of the ex- perimental period. The hippocampal neurons were observed by transmission electron microscope. Results: Compared with control group（343.28±10.41 g）, body weight of HF（356.81±36.20 g）, HF + VD rats（360.18±25.56 g） increased and that of DM rats (265.6±16.11 g)decreased（P>0.05）. SDF-1 decreased in diabetic rats（398.33±15.01 ng/L）. calcitriol treatment reversed the changes significantly （448.54±36.83 ng/L）（P<0.05）. 25-OH-D decreased in DM rats （165.25±30.75 μg/L）and calcitriol treatment increased 25-OH-D in DM rats 210.31±50.69 μg/L（P<0.05）. The expression of CXCR4 decreased in hippocampal neurons of DM rats, and swelled mito- chondria was observed in the neurons. calcitriol treatment improved the injury. Conclusion: calcitriol treatment increased vitamin D level, and has protective effect on hippocampal neurons in type 2 diabetes mellitus rats. |
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