| 夏 亮,刘江伟,沈才福,李佳佳,肇寅辉,宋来阳,是文辉,许 琴.姜黄素预处理通过保护线粒体和抗氧化应激减轻干热环境热射病大鼠肝损伤[J].现代生物医学进展英文版,2017,17(18):3434-3437. |
| 姜黄素预处理通过保护线粒体和抗氧化应激减轻干热环境热射病大鼠肝损伤 |
| Curcumin Pretreatment Alleviates the Liver Injury induced by Heatstroke in Dry-heat Environment through Protecting the Mitochondria and Inhibiting Oxidative Stress |
| Received:December 14, 2016 Revised:January 10, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.18.007 |
| 中文关键词: 姜黄素 干热环境 热射病 肝损伤 氧化应激 细胞色素C |
| 英文关键词: Curcumin Dry-heat environment Heatstroke Liver injury Oxidative stress Cytochrome-c |
| 基金项目:军队临床高新技术重大项目（2010gxjs016）；军队后勤科研计划面上项目（CLZ13J003） |
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| 中文摘要: |
| 摘要 目的：研究姜黄素预处理对干热环境热射病大鼠肝脏的保护作用及机制。方法：将50只SD大鼠随机分为5组(n=10)：常温对照组(Control)，干热对照组(HS)，50-cur组，100-cur组和200-cur组。Control组、HS组给予生理盐水灌胃，50-cur组、100-cur组、200-cur组分别给予50 mg/kg、100 mg/kg、200 mg/kg浓度的姜黄素灌胃，每天1次，连续7天。第8天除Control组外，其余4组大鼠转移至西北特殊环境人工实验舱(环境温度41±0.5 ℃，湿度10±1 %)进行实验。实验的第150分钟，检测肛温，麻醉后取材。同时Control组直接检测肛温并麻醉取材。检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的含量和细胞色素C(Cyt-c)的表达情况。电镜观察肝细胞超微结构的变化。结果：(1)第150分钟，各组大鼠体温均超过42 ℃，达到热射病状态。姜黄素预处理组体温较HS组降低(P<0.01)。姜黄素预处理组组间差异无统计学意义(P>0.05)。(2)HS组ALT、AST水平较Control组升高(P<0.01)。姜黄素预处理组ALT、AST水平较HS组降低(P<0.05)，姜黄素预处理组组间差异有统计学意义(P<0.05)。(3)HS组与Control组相比MDA水平升高，SOD、CAT水平降低(P<0.01)。姜黄素预处理组与HS组相比MDA水平降低，SOD、CAT水平升高(P<0.01)，姜黄素预处理组组间差异有统计学意义(P<0.05)。(4)电镜下，HS组肝细胞内线粒体增生肿胀，嵴结构紊乱、破坏，部分肝细胞核结构破坏；姜黄素预处理组肝细胞内线粒体增生为主，无嵴结构破坏和胞核的改变。(5)HS组肝细胞质内Cyt-c表达(1.29±0.19)较Control组(0.24±0.02)明显增多(P<0.01)。姜黄素预处理组Cyt-c表达(50-cur、100-cur、200-cur分别为0.75±0.08、0.64±0.08、0.48±0.06)较HS组相对减低(P<0.05)，姜黄素预处理组组间差异有统计学意义(P<0.05)。结论：干热环境热射病大鼠肝脏损伤明显，姜黄素预处理能减轻干热环境热射病导致的肝损伤，其机制可能与保护线粒体和抗氧化应激有关。姜黄素对热射病大鼠肝脏的保护作用具有剂量依赖性。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the positive effects of curcumin pretreatment to liver injury and the mechanism during heatstroke in dry-heat environment. Methods: 50 Sprague-Dawley rats were randomly divided into five groups (n=10): Control, HS, 50-cur, 100-cur and 200-cur. The Control and HS group were given a gavage of normal saline, while 50-cur, 100-cur, 200-cur group were given a gavage of curcumin with the concentration 50 mg/kg, 100 mg/kg, 200 mg/kg once a day for 7 consecutive days. At 8th day, all except Control group were transferred to the cabin (The Simulated Climate Cabin for Special Environment of Northwest of China, Urumqi of China) with the conditions of 41±0.5℃ temperature, 10±1 % relative humidity. At the time of 150th minute since the experiment began, the rats were in heatstroke, then be detected core temperature and anesthetized for collecting blood and liver samples. And same done to Control group. Alanine aminotransferase (ALT), aspartate aminotransferase (AST) in the blood, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and cytochrome-c (Cyt-c) in the liver were detected, ultrastructural changes of liver were observed by electron microscope. Results: (1) Tc of all rats in the cabin had risen to above 42 ℃ and conformed to the standard of heatstroke. Compared with HS group, Tc of curcumin pretreatment groups decreased (P<0.01). There were no significant differences among curcumin pretreatment groups (P>0.05). (2) Compared with Control group, ALT and AST of HS group increased (P<0.01). Compared with HS group, curcumin pretreatment decreased the levels of ALT and AST (P<0.05). The differences among curcumin pretreatment groups were significant (P<0.05). (3) Compared with Control group, MDA increased and SOD and CAT decreased in HS group (P<0.01). Compared with HS group, MDA decreased and SOD and CAT increased in curcumin pretreatment groups (P<0.01). The differences among curcumin pretreatment groups were significant (P<0.05). (4) Under the electron microscope，mitochondria swelled and proliferated with structure damaged and part of nucleus broken in HS group, while in 50-cur group, part of mitochondria swelled, but none of structure damaged, and in 100-cur and 200-cur group, only mitochondria proliferated obviously. (5) Compared with Control group (0.24±0.02), Cyt-C expressed high in HS group (1.29±0.19) (P<0.01). Curcumin pretreatment decreased the expression of Cyt-c (the levels of Cyt-c in 50-cur, 100-cur, 200-cur were 0.75±0.08, 0.64±0.08, 0.48±0.06) in the liver (P<0.05). The differences among curcumin pretreatment groups were significant (P<0.05). Conclusion: The liver injured during heatstroke in dry-heat environment and curcumin pretreatment could alleviate the liver injury through protecting mitochondria and inhibiting oxidative stress，and the protective effect was dose-dependent. |
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