| 王振东,赵雪琴,郭蒲君,周 琳,夏彦民,姜 涛.DDX5、E-cadherin在非小细胞肺癌中的表达及其临床意义[J].现代生物医学进展英文版,2017,17(17):3320-3324. |
| DDX5、E-cadherin在非小细胞肺癌中的表达及其临床意义 |
| The Expressions and Clinical Significances of DDX5 and E-cadherin in the Non-small Cell Lung Cancer |
| Received:December 03, 2016 Revised:December 26, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.17.030 |
| 中文关键词: 非小细胞肺癌 DDX5 E-钙黏蛋白 |
| 英文关键词: Non-small cell lung cancer (NSCLC) DDX5 E-cadherin |
| 基金项目:陕西省自然科学基础研究计划项目（2014JM2-8144） |
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| 中文摘要: |
| 摘要 目的：探讨DEAD-box家族的DDX5(RNA解旋酶)和E-钙黏蛋白(E-cadherin)在非小细胞肺癌(NSCLC)组织中表达，并分析其临床病理意义。方法：采用免疫组织化学方法(SP法)和Western blot法检测手术切除的NSCLC组织74例及癌旁组织 (距肿瘤>5 cm) 36例中DDX5和E-cadherin的表达情况，并对其与NSCLC患者的临床病理特征的相关性进行统计学分析。结果：NSCLC组织中DDX5的阳性表达率显著高于癌旁组织(63.5 % vs 30.6 %)， E-cadherin的阳性表达率显著低于癌旁组织(60.8 % vs 100 %)，差异均具有统计学意义(P<0.05)；DDX5和E-cadherin蛋白的表达水平与NSCLC的TNM分期以及淋巴结是否转移具有显著相关性(P<0.05)，但二者与NSCLC患者的年龄、性别和肿瘤组织类型均无显著相关性(P>0.05)。DDX5和E-cadherin蛋白的表达呈显著负相关(r=-0.327，P<0.05)。结论：DDX5的过度表达及E-cadherin的表达下调可能参与了NSCLC的发生发展，且二者在其中可能也具有相互作用的关系。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expressions and clinical significances of DEAD-box RNA helicase 5(DDX5) and E-cad- herin in the non-small cell lung cancer(NSCLC). Methods: The expressions of DDX5 and E-cadherin were measured with immunohisto- chemistry technique and western blot in 74 cases of non-small cell lung cancer tissues and 36 cases of adjacent normal tissues (5 cm away from carcinoma tissues) resected by surgery. The correlation of DDX5 and E-cadherin expressions and the clinical pathological features of NSCLC were also analyzed. Results: The expression of DDX5 was higher in NSCLC compared with the adjacent normal tissues(63.5% vs 30.6%)(P<0.05). The expression of E-cadherin was lower in NSCLC compared with the adjacent normal tissues (60.8% vs 100%)(P<0.05). The expressions of DDX5 and E-cadherin in NSCLC were related to the lymph node metastasis and pathological stage of the tumor(P<0.05). But there was no significant correlation with the age, sex and pathological type of NSCLC(P>0.05). A negative corre- lation was found between the expression of DDX5 and E-cadherin(r=-0.327, P<0.05). Conclusion: Overexpression of DDX5 and down-regulation of E-cadherin might be involved in the development of NSCLC, DDX5 and E-cadherin might interact mutually. |
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