| 闫 涛,王琴会,姜 维,陈 曦,赵 美,刘新友.在体单向肠灌流模型研究白芷中三种成分的吸收特性[J].现代生物医学进展英文版,2017,17(17):3238-3242. |
| 在体单向肠灌流模型研究白芷中三种成分的吸收特性 |
| Intestinal Absorption of Three Components in Angelicae Dahuricae by in situ Single Pass Intestinal Perfusion Model in Rat |
| Received:December 10, 2016 Revised:December 30, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.17.009 |
| 中文关键词: 白芷 在体单向肠灌流 高效液相色谱 肠吸收 |
| 英文关键词: Angelicae dahuricae In situ single pass perfusion model HPLC Intestinal absorption |
| 基金项目:陕西省中医药管理局资助项目（13-ZY039） |
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| 中文摘要: |
| 摘要 目的：研究白芷中有效成分欧前胡素、异欧前胡素和花椒毒酚在大鼠小肠各段的吸收特征。方法：采用大鼠在体单向肠灌流模型结合HPLC法同时测定肠灌流液中欧前胡素、异欧前胡素和花椒毒酚含量的变化，考察高、中、低三组剂量下三种成分在十二指肠、空肠和回肠的吸收特性。结果：三种成分随着剂量的增大，吸收速率常数(K a )和有效渗透系数(P eff )逐渐增大。三种成分相同剂量下各肠段间吸收均无显著性差异，其吸收大小顺序为：欧前胡素>异欧前胡素>花椒毒酚。结论：白芷三种主要成分在各肠段均吸收良好，吸收大小呈剂量相关性。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate absorption mechanism of imperatorin, isoimperatorin and methoxsalen in radix of Angelicae dahuricae. Methods: In situ single pass intestinal perfusion model was established, HPLC was used to determine the change of the con- centration of imperatorin, isoimperatorin and xanthotoxol in different segments. The absorption parameters of these three analytes in dif- ferent intestinal segments were investigated. Results: With the increasing of doses for these three analytes, the absorption rate constant (K a ) and effective permeability coefficient (P eff ) were increased. There were no significant differences in different segments of intestine in rat. The absorption order was imperatorin, isoimperatorin and xanthotoxol. Conclusion: Radix of Angelicae dahuricae was easily ab- sorbed in the intestine, the degree of absorption was dose-dependent. |
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