Article Summary
赵 黎,孙 媛,岳 媛,刘婷婷,姜 珊,高卫华.PKC βII特异性抑制剂LY333531减缓心肌梗死后心肌纤维化[J].现代生物医学进展英文版,2017,17(17):3234-3237.
PKC βII特异性抑制剂LY333531减缓心肌梗死后心肌纤维化
PKCβII Selective Inhibitor LY333531 Attenuates Myocardial Infarction Induced Fibrosis
Received:October 09, 2016  Revised:November 17, 2016
DOI:10.13241/j.cnki.pmb.2017.17.008
中文关键词: PKCβII  LY333531  心脏重塑  心肌梗死  心力衰竭  心肌纤维化
英文关键词: PKCβII  LY333531  Cardiac remodeling  Myocardial infarction  Heart failure  Myocardial fibrosis
基金项目:国家自然科学基金项目(81300956);陕西省自然科学基础研究计划面上项目(2016JM3005)
Author NameAffiliationE-mail
赵 黎 西安市第一医院心内科 陕西 西安 710002 2309391374@qq.com 
孙 媛 西安市第一医院心内科 陕西 西安 710002  
岳 媛 西安市第一医院心内科 陕西 西安 710002  
刘婷婷 西安市第一医院心内科 陕西 西安 710002  
姜 珊 西安市第一医院心内科 陕西 西安 710002  
高卫华 西安市第一医院心内科 陕西 西安 710002  
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中文摘要:
      摘要 目的:探讨蛋白激酶CβII(PKCβII)特异性抑制剂LY333531对心梗后大鼠心功能和心肌纤维化的保护作用。方法:利用左冠状动脉降支(LAD)结扎制备大鼠心梗模型,将手术4周后出现心衰的大鼠分为模型组(MF+NS)和治疗组(MF+LY333531),分别给予生理盐水和LY333531(10 mg/kg/d)处理6周,检测各组大鼠体重和各项心功能指标,利用HE染色观察各组大鼠心肌组织形态变化,利用天狼星红染色观察心肌组织胶原沉积情况。结果:相对于模型组,LY333531处理组大鼠左心室缩短率(FS)明显改善(从21 %到35 %)。HE染色显示LY333531能够部分逆转心衰大鼠心室壁肥厚和心肌细胞宽度,天狼星红染色显示治疗组胶原蛋白沉积降低150 %。结论:使用LY333531选择性抑制PKCβII能够改善心梗后心力衰竭模型大鼠心脏功能和抑制心肌纤维化。
英文摘要:
      ABSTRACT Objective: To investigate the protective effects of protein kinase C βII (PKCβII) selective inhibitor LY333531 on the myocardial fibrosis after myocardial infarction (MI). Methods: The left anterior descending (LAD) coronary artery of male Sprague-Daw- ley (SD) rats was occluded to induce MI. At four weeks after MI, rats with heart failure (HF) signs were treated with the LY333531 (3 mg/kg/day) or normal sodium (NS) for 6 weeks. The body weight and cardiovascular parameters were detected. Cardiomyocyte hypertro- phy was evaluated by haematoxylin and eosin (H&E) staining. Collagen deposition was evaluated by picrosirius red staining. Results: LY333531 treatment improved fractional shortening (from 21 % to 35 %) compared to control (NS). Mid-ventricle tissue sections stained with (H&E) showed that LY333531 treatment reduced wall thickness and cardiomyocyte width. Picrosirius red staining exhibited a 150 % decrease in collagen deposition in rat hearts treated with LY333531. Conclusion: Sustained selective inhibition of LY333531 in a post-MI HF rat model improves cardiac function and is associated with inhibition of pathological myocardial remodeling.
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