| 卫 国,殷 英,段佳林,梁凌飞,文爱东.丹红注射液中5种主要活性成分促血管新生作用研究[J].现代生物医学进展英文版,2017,17(17):3230-3233. |
| 丹红注射液中5种主要活性成分促血管新生作用研究 |
| Study on Proangiogenic Activities of Five Principal Active Components in Danhong Injection |
| Received:December 08, 2016 Revised:December 29, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.17.007 |
| 中文关键词: 丹红注射液 血管新生 人脐静脉内皮细胞 鸡胚绒毛尿囊膜 活性成分筛选 |
| 英文关键词: Danhong injection Angiogenesis Human umbilical vein endothelial cells Chick chorioallantoic membrane Screening of active components |
| 基金项目:国家自然科学基金项目（81403135） |
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| 中文摘要: |
| 摘要 目的：研究丹红注射液（DHI）入血后5个主要成分的促血管新生活性。方法：采用人脐静脉内皮细胞（HUVEC）正常培养体系和鸡胚绒毛尿囊膜（CAM）模型评价。细胞和鸡胚分别随机分为正常对照组，DHI组、羟基红花黄色素A组、丹酚酸B组、丹参素组，原儿茶醛组和迷迭香酸组。MTT法检测HUVEC增殖情况，免疫印迹法测定HUVEC促血管新生相关因子的表达，显微镜下计数鸡胚CAM给药区域新生血管数。结果：与正常对照组比较，10 %（V/V）DHI及相应浓度的各单体均能显著增加HUVEC的增殖（P<0.01），并促进HUVEC中促血管新生因子VEGF-A，bFGF，HGF的表达（P<0.05，P<0.01）；而50 %（V/V）DHI及相应浓度的各单体均能不同程度地增加鸡胚CAM给药区域新生血管数；两个筛选模型中，羟基红花黄色素A和丹参素均呈现一定的作用优势。结论：DHI入血后的5个主要活性成分都有促血管新生作用，其中羟基红花黄色素A和丹参素活性更佳。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the proangiogenic activities of five main components from Danhong injection (DHI) absorbed in blood. Methods: A in vitro culture system of human umbilical vein endothelial cells (HUVEC) and a model of chicken embryo chorioal- lantoic membrane (CAM) were used in this study. Cells or chicken embryos were randomly divided into control (Con) group, DHI group, hydroxysafflor yellow A (HSYA) group, salvianolic acid B (Sal B) group, Tanshinol (Tan) group, protocatechuic aldehyde (Pra) group and Rosmarinic acid (RosA) group. Results: In comparison with Con group, 10 %（V/V）DHI and its five main components at corre- sponding concentrations could significantly promote HUVEC proliferation (P<0.01) and the expression of vascular endothelial growth factor A (VEGF-A), basic fibroblast growth Factor (bFGF) and hepatocyte growth factor (HGF) in HUVEC (P<0.05, P<0.01). Also, 50 %（V/V）DHI and these components at corresponding concentrations were able to boost the blood vessel density on CAM to different ex- tent. Consistently, HSYA and Tan showed better proangiogenic action in both models. Conclusion: All the five main components from Danhong injection (DHI) absorbed in blood promote angiogenesis and HSYA and Tan have stronger proangiogenic activities than the others. |
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