| 董广璐,马艳伟,徐 军,金 茜,李洪佳.Egr-1基因沉默对A549细胞放射敏感性的影响[J].现代生物医学进展英文版,2017,17(16):3008-3011. |
| Egr-1基因沉默对A549细胞放射敏感性的影响 |
| Effects of Early Growth Response -1(EGR-1) Gene Silence on the Radiosensitivity of A549 Cell |
| Received:October 18, 2016 Revised:November 15, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.16.003 |
| 中文关键词: Egr-1 放射敏感性 细胞凋亡 A549细胞 |
| 英文关键词: Egr-1 gene Radiosensitivity Apoptosis A549 cell |
| 基金项目:黑龙江省教育厅科学技术研究项目（12521311） |
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| 中文摘要: |
| 摘要 目的：探讨Egr-1基因沉默对人肺腺癌A549细胞放射敏感性的影响。方法：选用A549细胞株作为研究对象，将其分成A、B、C三组，即空白对照组(只加入RPMI-1640培养)、阴性对照组(加入LV3-NC-shRNA)、实验组(加入EGR1-homo-2294-shRNA)，采用慢病毒介导的shRNA干扰技术使实验组细胞Egr-1基因沉默表达。利用荧光显微镜、自动化荧光定量细胞成像分析系统分析shRNA转染，利用FQ-PCR分析Egr-1表达，再利用细胞克隆形成实验检测细胞放射敏感性参数的差异。结果：0.05)，实验组与空白对照组、阴性对照组比较Egr-1均受到明显抑制(P<0.05)；克隆形成实验中细胞放射敏感性参数D0、SF2实验组细胞与空白对照组、阴性对照组比较均存在明显差异(P<0.05)。结论：Egr-1基因沉默使A549细胞放射敏感性降低，Egr-1表达可能与肿瘤细胞对放射的敏感性有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of early growth respons-1 (Egr-1) gene silence on the radiosensitivity of human lung adenocarcinoma A549 cells. Methods: The A549 cell line was selected and divided into the blank control group (only to join the RPMI-1640 Culture), the negative control group (to join LV3-NC-shRNA), the experimental group (to join EGR1-homo-2294-shRNA), Egr-1 gene expression was silenced in the experimental group with the ShRNA interference technique mediated by lentiviral vector. ShRNA transfection was analyzed by fluorescence microscopy and automated fluorescence quantitative cell imaging analysis system. The expression of Egr-1 was analyzed by FQ-PCR. And the difference of parameters of cell radiation sensitivity was detected by cell cloning experiment. Results: The negative control group and the experimental group cells were successfully transfected with the shRNA by lentiviral vector. There was no difference in the Egr-1 expression between the blank control group and the negative control group (P>0.05). Compared with the blank control group and negative control group, the Egr-1 expression was significantly inhibited (P<0.05), the cell radiosensitivity parameters of D0 and SF2 in the clone formation experiment also showed significant differences in the experimental group(P<0.05). Conclusion: Egr-1 gene silence reduced the radiosensitivity of A549 cells and the expression of Egr-1 was correlated with the radiosensitivity of tumor cells. |
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