| 曹昆昆,章建全,赵佳琦,黄禾菁,吴震中.透明质酸钠预防微波消融术后局部粘连的实验研究[J].现代生物医学进展英文版,2017,17(15):2837-2842. |
| 透明质酸钠预防微波消融术后局部粘连的实验研究 |
| Experimental Study of Sodium Hyaluronate in Preventing Adhesion after Microwave Ablation |
| Received:November 28, 2016 Revised:December 30, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.15.010 |
| 中文关键词: 透明质酸钠 微波消融 粘连 预防 |
| 英文关键词: Sodium hyaluronate Microwave ablation Adhesion Preventing |
| 基金项目:国家自然科学基金项目（81171436） |
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| 中文摘要: |
| 摘要 目的：通过实验探讨透明质酸钠预防微波消融术后局部组织粘连的效果。方法：120只健康SD大鼠随机分为三组，每组40只，分别为微波消融组（A组，对照组）、微波消融加透明质酸钠组（B组）、微波消融加生理盐水组（C组）。通过对大鼠背部肌肉进行微波消融建立术后局部粘连模型，并在该模型基础上应用透明质酸钠，术后3 d、1 w、2 w、4 w分别对背部组织进行粘连分级、HE染色及免疫组化观察和分析。结果：消融术后3 d、1 w、2 w，B组在粘连分级方面A、B及C 3组间水平差异具有统计学意义P＜0.05)；4 w时A、B、C组间的差异无统计学意义（P=0.458）。两两比较，A、C两组在四个时间点水平差异无统计学差异（P均＞0.05），而B组在消融术后3 d、1 w和2 w时，粘连分级明显优于A、C组，P=0.029（3 d）、P=0.011（1W）、P=0.004（2W），水平差异显著，有统计学意义；4 w时，P=0.391，无统计学意义。消融术后A、C组HE染色及免疫组化染色光镜下观察，淋巴细胞、巨噬细胞等炎症浸润情况及成纤维细胞数目均较B组多，且A、C组胶原纤维沉积明显多于B组。结论：透明质酸钠能够有效减轻微波消融术后发生的局部组织粘连。 |
| 英文摘要: |
| ABSTRACT Objective: To determine the effectiveness of sodium hyaluronate in preventing local tissue adhesion after microwave ablation. Methods: 120 rats were randomly divided into three groups(40 rats in each group): microwave ablation group (group A), microwave ablation and sodium hyaluronate group (group B), microwave ablation and sodium hyaluronate saline group (group C). The local adhe- sion model was established in muscle of rat back by microwave ablation, and sodium hyaluronate was applied in this model during opera- tion. Biopsy was taken on the 3th, 7th, 14th and 28th postoperative day. The adhesion scores gradation judging, hematoxylin and Im- munohistochemistry were observed and analyzed. Results: There were statistically significantly differences in the extent of adhesion be- tween group B and group A, C on the 3th, 7th and 14th postoperative(P<0.05), after ablation 4W, there was not statistically signifi- cantly differences among A, B, C three groups (P=0.458). Comparison between the two group, there was no statistical significance be- tween the group A and C in the four time points (P>0.05), however, on the 3th, 7th and 14th postoperative day, group B were significantly less than group A , C in the extent of adhesion, and there was statistical significance, P=0.029(3d), P=0.011(1w), P=0.004(2w). After ablation 4w, P=0.391, there was no statistical significance between group B and group A, C.Group A and C were more than group B in the aspect of hematoxylin and immunohistochemical staining, the number of inflammatory cells infiltrated by lymphocytes, fibroblasts and macrophages, and the amount of collagen fiber deposition in A and C group were significantly harder than group B. Conclusion: Sodium hyaluronate appears to effectively reduce the local tissue adhesion after microwave ablation. |
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