| 杨思聪,刘海军,董 霄,陆 琴,方 超.pH敏感纳米粒的制备和功能表征[J].现代生物医学进展英文版,2017,17(15):2808-2812. |
| pH敏感纳米粒的制备和功能表征 |
| Synthesis and Characterization of pH Sensitive Nanoparticles |
| Received:December 13, 2016 Revised:January 10, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.15.003 |
| 中文关键词: ATRP聚合反应 pH响应 纳米粒 荧光探针 |
| 英文关键词: ATRP (Atom Transfer Radical Polymerization) pH responsive Nanoparticle Fluorescence probe |
| 基金项目:国家自然科学基金项目（81272569） |
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| 中文摘要: |
| 摘要 目的：制备具有pH响应的甲氧基聚乙二醇甲基丙烯酸-2-六亚甲基亚胺乙酯聚合物，测试材料pH功能响应，以及建立聚合物纳米粒载药方法。方法：通过核磁共振氢谱鉴定ATRP(Atom Transfer Radical Polymerization)聚合反应所获得的化合物结构。滴加-搅拌挥发法制备聚乙二醇甲基丙烯酸-2-六亚甲基亚胺乙酯纳米粒，酶标仪测定其载药量和包封率。透射电镜下观察其形态，激光粒度仪分析测定其粒径，包载DiR红外荧光探针检测纳米粒pH响应功能。结果：分别成功合成得到2-溴代异丁酸聚乙二醇单甲醚和甲基丙烯酸-2-六亚甲基亚胺乙酯单体。通过ATRP聚合反应成功合成聚乙二醇甲基丙烯酸-2-六亚甲基亚胺乙酯聚合物材料，并通过核磁氢谱对聚合材料进行鉴定。通过滴加搅拌法制备包载有模型药物香豆素-6的纳米粒，并对纳米粒的形态表征及载药量进行测定。结论：试验结果表明制备得到的聚合物纳米粒尺寸均匀，具有预期的pH响应效果，可以装载模型药物。 |
| 英文摘要: |
| ABSTRACT Objective: Prepare pH sensitive PEG-C7AMA polymer material. Test polymer nanoparticle pH functional property and establish drug loading method. Methods: The 1HNMR is used to detect the polymer materials obtained from ATRP (Atom Transfer Radi- cal Polymerization) polymerization reaction. Use Drop-stir evaporation method to prepare PEG-C7AMA nanoparticle and microplate reader to detect nanoparticle drug loading and entrapping efficiency. Use TEM (Transmission Electron Microscope) & DLS (dynamic light scattering) to observe and analyze nanoparticle shape respectively. Polymer nanoparticle load DiR fluorescence probe is used to de- tect pH functional property with microplate reader. Results: Success to synthesis PEG macroinitiator and 2-azepane ethyl methacrylate re- spectively, which have been detected by 1HNMR spectrum. Success to synthesis PEG-C7AMA polymer material through ATRP poly- merization reaction and 1HNMR to detect polymer materials. Through solvent-evaporation method to prepare nanoparticle which have been loaded coumarin-6, as a model drug, and to detect drug loading, entrapping efficiency and the shape of the established nanoparticle. Conclusion: Through experimental results show that the size of nanoparticle is on average. Nanoparticle have anticipated pH functional effect and can load model drug. |
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