| 肇寅辉,敖 其,刘江伟,李建瑛,张东辉,是文辉,夏 亮.姜黄素预处理对干热环境下中暑大鼠肾组织NF-κB p65及炎症因子表达的影响[J].现代生物医学进展英文版,2017,17(14):2622-2626. |
| 姜黄素预处理对干热环境下中暑大鼠肾组织NF-κB p65及炎症因子表达的影响 |
| The Effect of Curcumin on the Expressions of Inflammatory Factors and NF-κB p65 in the of Dry-heat Environment Induced Rats |
| Received:December 06, 2016 Revised:December 26, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.14.005 |
| 中文关键词: 急性肾损伤 干热环境 姜黄素 炎症反应 核因子-κB |
| 英文关键词: Acute renal injury Dry-heat environment Curcumin Inflammatory reaction |
| 基金项目:军队临床高新技术重大项目(2010gxjs016)；军队后勤科研计划面上项目（CLZ13J003） |
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| 中文摘要: |
| 摘要 目的：探讨不同剂量姜黄素对沙漠干热环境下大鼠肾组织炎症因子及NF-κB p65表达的影响。方法：将50只SD大鼠随机(随机数字法)分为五组(n=10)：常温对照组、干热对照组、姜黄素低浓度组(50 mg/kg)、姜黄素终浓度组(100 mg/kg)、姜黄素高浓度组(200 mg/kg)。常温对照组及干热对照组均给予0.9 %生理盐水灌胃，姜黄素组大鼠给予不同浓度姜黄素溶液灌胃，连续7天。在干热环境中放置150分钟后，麻醉处死大鼠，留取血液、肾组织进行分析。观察各组大鼠肾组织形态，检测肾组织中IL-1β、IL-6、TNF-α以及NF-κB p65表达及血清肌酐、尿素氮以及尿液中肾损伤分子1(KIM-1)、中性粒细胞明胶酶相关载脂蛋白(NGAL)的水平。结果：干热对照组大鼠血清肌酐、尿素以及尿液中KIM-1、NGAL水平均较常温对照组明显升高(P<0.05)，不同浓度姜黄素预处理组大鼠血清肌酐、尿素以及尿液中KIM-1、NGAL均较干热对照组有所下降，且差异具有统计学意义（P<0.05）。干热对照组大鼠肾组织中IL-1β、IL-6、TNF-α以及NF-κB p65、磷酸化IκB-α的表达较常温对照组显著增加(P<0.05)。姜黄素处理组大鼠随着姜黄素浓度的升高各指标较干热对照组呈不同程度的下降趋势（P<0.05）。结论：姜黄素对于干热环境下大鼠肾损伤的保护作用，可能是与抑制NF-κB的表达降低细胞炎症反应水平有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the influence of different doses of curcumin on the inflammatory factors and the expression of NF-κB p65 in kidney tissue of rats in the dry-heat environment. Methods: 50 SD rats were randomly divided into five groups (n = 10): normal temperature control group, dry-heat control group and curcumin pretreated groups including low dose (50 mg/kg), moderate dose(100 mg/kg), high dose(200 mg/kg) groups. The two control groups were given 0.9 % saline by gavage, the curcumin group were given different concentrations of curcumin solution by gavage. All the rats were treated for seven consecutive days. The curcumin was dissolved in 0.5% sodium carboxymethylcellulose(CMCNa) solution. The rats were put in the dry-heat environment for 150 min, anaesthetized and sacrificed at 150min. The pathological changes of renal tissue and the changes of the inflammatory factors such as IL-1β, IL-6, TNF-α in the renal tissue and kidney injury molecule 1 (KIM-1), neutrophil gelatinase lipoprotein (NGAL) of urine were observed. Meanwhile, the expression of NF-κB p65 in renal tissue were observed. Results: Compared with the normal temperature group, the creatinine, blood urea nitrogen (BUN) in the serum and Kidney Injury Molecule-1 (KIM-1), Neutrophil gelatinase-associated lipocalin(NGAL) in the urine were significantly increased in the dry-heat control group (P<0.05). However, with the pretreatment of different doses of curcumin, the serum BUN, Cr and KIM-1, NGAL in the urine showed downward trend compared with the dry-heat control group(P<0.05). The flammatory factors IL-1β, IL-6, TNF-α in the kidney tissue were also significantly increased in the dry-heat control group compared with the normal control group（P<0.05）, with the pretreatment of different dose of curcumin, the IL-1β, IL-6, TNF-α of kidney tissue were decreased compared with the dry-heat control group（P<0.05）. Meanwhile, the expressions of NF-κB p65 and phosphorylated IκB-α in kidney tissue were also increased in the normal temperature control group(P<0.05). The expression of NF-κB p65 and phosphorylated IκB-α level were declined in the curcumin pretreated groups(P<0.05). Conclusion: Curcumin could obviously reduce the renal inflammation and pathological changes in the rat under dry-heat environment, which might be related to the inhibition of the NF-κB p65 expression and inflammatory factors in the kidney. |
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