| 刘少鹏,郑立跃,康晓宇,潘阳林,郭学刚.SNFYMPL荧光探针和共聚焦显微内镜在胃癌诊断中的应用[J].现代生物医学进展英文版,2017,17(13):2450-2452. |
| SNFYMPL荧光探针和共聚焦显微内镜在胃癌诊断中的应用 |
| Application of SNFYMPL Fluorescence Probe in Diagnosis of Gastric Cancer Using Confocal Laser Endomicroscopy |
| Received:November 29, 2016 Revised:December 23, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.13.012 |
| 中文关键词: SNFYMPL 短肽探针 共聚焦显微内镜 胃癌 |
| 英文关键词: SNFYMPL Oligopeptide probe Confocal laser endomicroscopy Gastric cancer |
| 基金项目:国家自然科学基金项目（81370585）；第四军医大学科技发展基金计划（4174122286） |
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| 中文摘要: |
| 摘要 目的：探讨荧光探针SNFYMPLGGGSK-FITC与胃癌组织的结合能力，预测其在胃癌诊断中的应用价值。方法：收集我院2015年6月至10月收治的48例胃癌患者手术切除的肿瘤和癌旁组织，使用异硫氰酸荧光素FITC标记的线性七肽SNFYMPL及无关序列肽探针对其冰冻切片进行荧光染色，通过共聚焦激光扫描显微镜CLSM检测其结合能力，并与组织病理结果对比。结果：SNFYMPLGGGSK-FITC对胃癌组织荧光染色的阳性率为81.25 %(39/48)，明显优于无关序列肽探针的14.58 %(7/48)，差异有统计学意义(P<0.001)。SNFYMPLGGGSK-FITC对癌旁组织荧光染色的阳性率为27.08 %(13/48)，明显低于对胃癌组织荧光染色阳性率，差异有统计学意义(P<0.001)。结论：FITC标记的SNFYMPL短肽探针与胃癌组织较强结合能力，图像组织分辨率好，可以作为共聚焦激光显微内镜在胃癌诊断的潜在靶向分子进一步研究。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the binding capacity of fluorescent probe SNFYMPLGGGSK-FITC to gastric cancer tissue, and its applying potential in the diagnosis of gastric cancer. Methods: 48 pairs of gastric cancer tissues and adjacent non-tumor tissues of operation-resected in our hospital from June to October in 2015 were collected. FITC labelled oligopeptide SNFYMPL and unrelated se- quence peptide probe were used to stain their cryosections. The binding capacity were examined with confocal laser scan microscope(CLSM). Pathological result were used as the standard control. Results: The positive ratio of SNFYMPLGGGSK-FITC stained gastric cancer tissues was 81.25 %(39/48), while the unrelated sequence peptide probe had a much lower positive ratio (14.58 %, 7/48) (P<0.001). The positive ratio of SNFYMPLGGGSK-FITC stained adjacent non-tumor tissues was also much lower (27.08 %, 13/48) (P<0.001). Conclusions: Conclusion: FITC labelled oligopeptide SNFYMPL had high affinity with gastric cancer tissues, and could be a potential tar- geting molecule for the application of confocal laser endomicroscopy in the diagnosis of gastric cancer. |
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