| 吴 佼,李 勇,南 刚,边惠洁,陈志南.荧光显微成像在医学细胞生物学实验教学中的应用探索[J].现代生物医学进展英文版,2017,17(12):2364-2367. |
| 荧光显微成像在医学细胞生物学实验教学中的应用探索 |
| Application of Fluorescence Micro-imaging System in Experimental Teaching of Medical Cell Biology |
| Received:September 22, 2016 Revised:October 16, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.12.042 |
| 中文关键词: 荧光显微镜 激光共聚焦显微镜 细胞增殖 细胞骨架 细胞凋亡 |
| 英文关键词: Fluorescence Microscope Laser scanning confocal microscopy Cell proliferation Cellular cytoskeleton Apoptosis |
| 基金项目:国家自然科学基金项目（31401222, 31401190） |
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| 中文摘要: |
| 摘要：医学细胞生物学是医学院校本科生的重要基础学科之一，其实验课程教学工作的改革与创新具有重要意义。本文深入分析了医学细胞生物学实验课程现状，认为课程普遍存在目的不明确、内容不新颖、缺乏整体性、临床联系少等问题。荧光显微成像技术是细胞生物学研究中的关键技术，利用这一技术设计具有整体性的科研实验对于培养医学生操作能力和科研素养具有重要意义。顺铂（cisplatin）为当前肿瘤联合化疗中最常用的药物之一，由于其抗癌机制涉及细胞增殖、细胞凋亡、细胞骨架等一系列细胞生物学知识，且与临床联系紧密，非常适合用于综合性实验设计。实验方案拟使用BrdU染色、Hoechst33258染色及鬼笔环肽染色法，分别检测人肺癌细胞系A549顺铂处理后细胞增殖、细胞凋亡及细胞骨架重排情况。实验完成后，鼓励学生通过查阅文献得出综合的实验结论。希望能够通过科研与教学相融合的方式，更好的激发医学生的学习热情，提高医学生的科研素质，培养全方位的医学人才。 |
| 英文摘要: |
| ABSTRACT: Medical cell biology is one of the most important basic subjects for undergraduate students in medical universities, and the reform and innovation of experiment course are of great significance. We analyzed the problems existing in the experiment course of medical cell biology. The experiment course lacks clear target, fresh content and integrality, and is not combined with clinical knowledge. The project of teaching reform using fluorescence micro-imaging system is presented based on subsistent problems in classical courses system. The fluorescence micro-imaging system is widely used for the study of cell biology, and we believed it necessary to design integral scientific research experiments for medical cell biology course based on this technique. Cisplatin is a chemotherapeutic drug that has become one of the most commonly used drugs against tumors, and cisplatin-based chemotherapy is always combined with other therapies. Cisplatin destroys tumor cells by binding to DNA strands, interfering with DNA replication. The mechanisms involved in cisplatin-mediated anti-cancer effect also include the induction of apoptosis and the remodeling of cellular cytoskeleton. Since these mechanisms are closely correlated with the knowledge in medical cell biology and clinic, it is very suitable to design an integral experiment involving cisplatin. We plan to determine the cell proliferation, apoptosis and cellular cytoskeleton remodeling of human lung cancer cell line A549 with cisplatin treatment by BrdU staining, Hoechst 33258 staining and phalloidin staining, respectively. After completion of the experiments, students should be encouraged to consult literature and draw comprehensive conclusion of the integral experiments. We hope the project that combines scientific research and teaching could stimulate students' enthusiasm, raise their research abilities and help to train qualified people to meet the needs of the country. |
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