| 窦春青,金 鑫,孙丽媛,韩明明,张 宝,王大东,王有龙,李 涛.肝癌组织中miR-338-3p的表达及与临床病理参数的关系[J].现代生物医学进展英文版,2017,17(12):2290-2293. |
| 肝癌组织中miR-338-3p的表达及与临床病理参数的关系 |
| Expression of miR-338-3p and its Relationship with Clinicopathological Parameters in Hepatocellular Carcinoma |
| Received:December 26, 2016 Revised:January 22, 2017 |
| DOI：10.13241/j.cnki.pmb.2017.12.022 |
| 中文关键词: 肝癌 组织 miR-338-3p 病理参数 临床意义 |
| 英文关键词: Hepatocellular carcinoma Tissue miR-338-3p Pathological parameters Clinical significance |
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| 中文摘要: |
| 摘要 目的：探讨肝癌组织中微小RNA-338-3p（miR-338-3p）的表达及与临床病理参数的关系。方法：选取2015年1月至2016年6月我院手术获得的67例肝癌组织标本，同时每例标本均取癌旁正常组织标本作为配对对照，采用实时定量逆转录聚合酶链反应（RT-qPCR）对两组组织标本中的miR-338-3p进行检测，并分析其与肝癌临床病理特征的关系。结果：45例（67.16%）miR-338-3p表达下调，22例（32.85%）表达上调；RT-qPCR结果显示，肝癌组织中miR-338-3p的相对含量为（0.76±0.38），低于癌旁正常组织中的（1.23±0.45），差异有统计学意义（t=-6.259，P=0.000）。miR-338-3p在低分化、TNM分期III+IV期、肿瘤浸润深度T3+T4期、有淋巴结转移肝癌患者肝癌组织中的表达下调率高于中高分化、I+II期、T1+T2期、无淋巴结转移肝癌患者，差异有统计学意义（P<0.05）。不同性别、年龄、病理类型、肿瘤大小肝癌患者肝癌组织中miR-338-3p表达下调率差异无统计学意义（P>0.05）。结论：miR-338-3P在肝癌组织中呈低表达水平，与分化程度、TNM分期、肿瘤浸润深度、淋巴结转移有关，可能参与了肝癌的发生发展过程，早期检测可作为评估肝癌病情的指标。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the expression of microRNA-338-3p (miR-338-3p) in hepatocellular carcinoma and its relationship with clinicopathological parameters. Methods: A total of 67 hepatocellular carcinoma tissue samples obtained by surgery from January 2015 to June 2016 in our hospital were selected, and adjacent normal tissue samples matched to each hepatocellular carcinoma tissue sample were selected. The miR-338-3p in tissue samples of the two groups were detected by the real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), and the relationship between miR-338-3p and clinicopathologic features of hepatocellular carcinoma was analyzed. Results: There were 45 cases (67.16%) of miR-338-3p down expression, 22 cases (32.85%) of up expression; RT-qPCR results showed that the relative content of miR-338-3p in hepatocellular carcinoma tissues was (0.76±0.38), lower than (1.23±0.45) in the adjacent normal tissues, the difference was statistically significant (t=-6.259, P=0.000). The expression down-regulation rate of miR-338-3p in hepatocellular carcinoma tissue samples of patients with poorly differentiated, III&IV stages of TNM, T3&T4 stages of tumor infiltrating depth, lymph node metastasis were higher than those with well differentiated, I&II stages, T1&T2 stages, without lymph node metastasis, the differences were statistically significant (P<0.05). The differences of the expression down-regulation rate of miR-338-3p in different gender, age, pathological type and tumor size were not statistically significant (P>0.05). Conclusion: MiR-338-3p expression was low in hepatocellular carcinoma tissues, which may be related to the degree of differentiation,TNM stage, depth of tumor invasion and lymph node metastasis. MiR-338-3p may be involved in the development and progression of hepatocellular carcinoma, and early detection of miR-338-3p can be used as an indicator to evaluate hepatocellular carcinoma condition. |
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