| 周 洁,黄星原,魏 丽,刘奥杰,陈娇娇.孟鲁司特钠对柯萨奇B3病毒感染小鼠心肌的保护作用[J].现代生物医学进展英文版,2017,17(12):2209-2213. |
| 孟鲁司特钠对柯萨奇B3病毒感染小鼠心肌的保护作用 |
| The Protective Effect of Montelukast Sodium on CBV3 Infection in Mice Myocardium |
| Received:September 20, 2016 Revised:October 10, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.12.003 |
| 中文关键词: 柯萨奇B3病毒 病毒性心肌炎 孟鲁斯特钠 核因子-κB |
| 英文关键词: Coxsackie B3 virus Viral myocarditis Montelukast sodium Nuclear factor-κB |
| 基金项目:湖北省科技攻关项目(301130801) |
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| 中文摘要: |
| 摘要 目的：探讨半胱氨酰受体拮抗剂孟鲁司特钠( montelukast)对于柯萨奇B3（CVB3）感染小鼠心肌损伤模型的保护作用。方法：将65只SPF级BALB/C小鼠，雄性，体重为16-20 g，随机分成四组：A组为空白对照组（10只）；B组为病毒对照组（15只）；C组为孟鲁司特钠小剂量组（20只）；D组为孟鲁司特钠大剂量组（20只）。在接种病毒第14天处死所有存活小鼠，取心脏，通过各种检测技术观察各组小鼠心肌病变积分、核因子-κB（NF-κB）的差异。结果：孟鲁司特钠治疗组心肌NF-κB mRNA及蛋白表达水平均低于病毒对照组（P <0 .05），且大剂量组较明显；同时孟鲁司特钠治疗组及病毒对照组心肌NF-κB mRNA及蛋白表达水平与心肌病变积分呈正相关（r=0.96，P <0 .05）。结论：孟鲁司特钠对心肌损伤小鼠血清中的核因子-kB（NF-κB）有抑制作用，尤其是大剂量组效果显著，孟鲁司特钠对CVB3感染小鼠心肌具有保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the cysteinyl aminoacyl receptor antagonist agent montelukast sodium (montelukast) for coxsackievirus B3 (CVB3) infection the protective effect of myocardial injury in mice model. Methods: 65 SPF BALB / c mice, male, weight is 16 to 20 gram were randomly divided into four groups: group a blank control group (10 rats); group B virus control group (15 rats); group C for montelukast sodium group of small dose (20 rats), group D for montelukast sodium group of large dose (20 rats). The inoculation of the virus on the 14th day were all surviving mice, take heart, differences in each group of mice the myocardial lesion score, nuclear factor kB (NF-κB) was observed through various detection technologies. Results: Montelukast sodium treatment group myocardial NF-κB mRNA and protein expression levels were lower than that of the virus control group (P < 0.05), and high dose group significantly; at the same time of montelukast sodium in treatment group and virus control group myocardial NF-κB mRNA and protein expression level and the myocardial lesion score was positively correlated (r = 0.77, P < 0.05). Conclusion: Montelukast sodium on myocardial injury in mice serum nuclear factor κB (NF-κB) inhibition, especially significant effect of high dose group and montelukast sodium on CVB3 infection of myocardium in mice has a protective effec. |
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