| 孟伟栋,徐 斌,刘潇潇,赵艳镯,秦春焕,王 毅,韩晓玲,李红莉.心肌梗死后心衰大鼠血液中同型半胱氨酸的变化及意义[J].现代生物医学进展英文版,2017,17(12):2205-2208. |
| 心肌梗死后心衰大鼠血液中同型半胱氨酸的变化及意义 |
| Change and Significance of Homocysteine in Heart Failure Post Myocardial Infarction in Rat |
| Received:September 28, 2016 Revised:October 20, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.12.002 |
| 中文关键词: 心力衰竭 同型半胱氨酸 脑钠肽 大鼠 |
| 英文关键词: Heart failure Homocysteine B-type natriuretic peptide Rat |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：研究同型半胱氨酸在心肌梗死后心衰大鼠血液中的变化及意义。方法：通过结扎大鼠左冠状动脉前降支的方法建立心梗后心衰动物模型。 成年雄性SD大鼠随机分为假手术对照组（Sham），心衰组（HF组），每组7只。对各组大鼠行心脏超声及血流动力学检查评价心功能，分别测定左室射血分数（Left ventricular ejection fraction, LVEF），左室短轴缩短率（left ventricular fractional shorting，LVFS），动脉收缩压（Systolic blood pressure， SBP），舒张压(Diastolic blood pressure，DBP)，左室舒张末压（Left ventricular end-diastolic pressure LVEDP）。上述检查完成后处死大鼠，收集血液标本检测脑钠肽（B-type natriuretic peptide，BNP），同型半胱氨酸（Homocysteine，Hcy），肌酐（Creatinine，Cr），尿酸（Uric Acid，UA），叶酸（Folic acid，Fa）水平；测定梗死周边区心肌组织超氧化物歧化酶（Superoxide dismutase，SOD）活性及丙二醛（Malondialdehyde，MDA）含量。结果： 与对照组相比，心衰组LVEF，LVFS显著降低；LVEDP增高；BNP、Hcy、Cr和UA水平比正常组升高，SOD活性明显下降，MDA含量明显升高（均P<0.05）。结论：同型半胱氨酸可能促使心肌梗死后心力衰竭发展。 |
| 英文摘要: |
| ABSTRACT Objective: To study change and significance of homocysteine in heart failure(HF) post myocardial infarction in rat. Methods: HF model was induced in Sprague Dawley (SD) rats by ligation of the left anterior descending coronary artery. Male SD rats were randomly divided into 2 groups: Sham group, HF group. Cardiac function was assessed by echocardiography and cardiac catheterization. Left ventricular ejection fraction (LVEF) and fractional shortening (LVFS) were calculated; systolic blood pressure (SBP), diastolic blood pressure (DBP), and left ventricular end-diastolic pressure (LVEDP) were recorded. After echocardiographic study and haemodynamic measurement, rats were killed and blood samples were collected to detect the levels of the B-type natriuretic peptide (BNP), Homocysteine（Hcy）, Creatinine（Cr）, Uric Acid（UA）, Folic acid（Fa）. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in peri-infarct zone were also examined. Results: In comparison to the normal group, HF significantly decreased the LVEF and LVFS（P<0.05）. The LVEDP in the HF group was higher than that in the normal group（P<0.05）. The plasma BNP, Hcy, Cr, UA levels were increased in the HF group（P<0.05）. Moreover, compared with the normal group, the SOD activity in the peri-infarct zone of HF group was significantly decreased, while the MDA level was significantly increased（P<0.05）. Conclusion: The homocysteine may promote heart failure development post myocardial infarction. |
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