| 蒲 猛,汪建林,黄启科,赵 戈,夏聪聪,何大立,杨 针,陶开山.Bub1基因对人肝癌MHCC97-H细胞增殖、周期和凋亡的影响[J].现代生物医学进展英文版,2017,17(12):2201-2204. |
| Bub1基因对人肝癌MHCC97-H细胞增殖、周期和凋亡的影响 |
| Influence of Bub1 Gene on the Cell Proliferation, Cell Cycle and Apoptosis in Human Hepatocellular Carcinoma Cell Line MHCC97-H |
| Received:October 10, 2016 Revised:November 05, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.12.001 |
| 中文关键词: 肝癌 Bub1 增殖 细胞周期 细胞凋亡 |
| 英文关键词: Hepatocellular carcinoma Bub1 Proliferation Cell cycle Apoptosis |
| 基金项目:国家自然科学基金项目（81670593；81272648） |
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| 中文摘要: |
| 摘要 目的：研究Bub1基因在肝癌中的表达以及对肝癌细胞系MHCC97-H增殖、周期和凋亡的影响。方法：利用RNA干扰技术下调肝癌细胞系MHCC97-H中Bub1的表达；qRT-PCR和Western Blot分别检测Bub1在mRNA和蛋白水平表达的变化；CCK-8实验检测肿瘤细胞增殖能力的改变；流式细胞术检测细胞周期和凋亡的变化。结果：qRT-PCR和Western Blot结果显示si-Bub1能够成功下调Bub1的表达；下调Bub1后肝癌MHCC97-H细胞的增殖能力下降(P<0.05)，细胞的凋亡比例升高(P<0.05)，细胞发生S期阻滞。结论：Bub1基因在肝癌中高表达，下调Bub1的表达后能够降低肝癌细胞的增殖能力，促进细胞凋亡，诱导细胞发生S期阻滞。 |
| 英文摘要: |
| ABSTRACT Objective: To study the expression of Bub1 in hepatocellular carcinoma (HCC) and its effect on the cell proliferation, cell cycle and apoptosis in hepatocellular carcinoma cell line MHCC97-H. Methods: Bub1 was down-regulated by small interfering RNA (siRNA) in MHCC97-H. qRT-PCR and Western blot were used to determine the expression of mRNA and protein. CCK-8 assay was performed to evaluate the proliferation ability. Apoptosis and cell cycles were determined by flow cytometry. Results: qRT-PCR and Western blot showed that si-Bub1 group displayed a low expression of Bub1. Down-regulated of Bub1 by siRNA significantly inhibited the cell proliferation (P<0.05), Cell cycle had changed and increased the cell apoptosis rates (P<0.05). Conclusion: Bub1 was highly expressed in hepatocellular carcinoma and down-regulating the expression of Bub1 could reduce the proliferation of hepatocellular carcinoma cells and affect the cell cycle and apoptosis of HCC. |
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