| 王珏云,赵公泽,白鑫洁,田聪阳,侠晨辉,李姝玉.柴胡解毒汤对实验性酒精性肝纤维化复合模型大鼠Col-IV，HA的影响[J].现代生物医学进展英文版,2017,17(11):2016-2019. |
| 柴胡解毒汤对实验性酒精性肝纤维化复合模型大鼠Col-IV，HA的影响 |
| Influence of Chaihu Jiedu Decoction on the Rat Model of Experimental Alcoholic Hepatic Fibrosis |
| Received:October 21, 2016 Revised:November 19, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.11.004 |
| 中文关键词: 柴胡解毒汤 酒精性脂肪肝肝纤维化 透明质酸(HA) IV型胶原(Col-IV) |
| 英文关键词: Chaihu Jiedu decoction Alcoholic fatty alcoholic hepatic fibrosis Hyaluronic Acid(HA) Collagen Type IV(Col-IV) |
| 基金项目:北京市大学生科学研究与创业行动计划项目（BJGJ1515） |
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| 中文摘要: |
| 摘要 目的：观察柴胡解毒汤对实验性酒精性肝纤维化复合模型大鼠Col-IV、HA血清含量的影响。方法：通过采用酒、猪油混合灌胃诱导大鼠实验性酒精性肝纤维化复合模型，并分为柴胡解毒汤干预I组（简称干预I组）、柴胡解毒汤兼酒精高脂饮食组（简称干预II组）、酒精兼高脂饮食组（简称模型组）及空白对照组予以灌胃，最终取大鼠血清进行检测。称量大鼠体重，并用ELISA Kit法检测透明质酸(HA)和IV型胶原（Col-IV）的含量。结果：12周后，造模各组大鼠毛发灰暗，杂乱，消瘦，活动度减弱，大便溏稀。血清Col-IV、HA较空白对照组均有显著升高（P＜0. 01），与模型组比较，干预I组和干预II组均下降明显（P＜0.01）。干预I组、干预II组、模型组、空白对照组HA含量依次为219.17±41.64、184.89±22.83、249.32±34.60、123.48±25.85。Col-IV含量依次为9.59±1.81、12.57±1.41、19.71±6.04、7.62±5.36。结论：柴胡解毒汤可能通过抑制Col-IV、HA的表达水平，对大鼠实验性酒精性肝纤维化复合模型具有一定的干预作用。 |
| 英文摘要: |
| ABSTRACT Objective: The paper aims to observe the effect of Chaihu Jiedu decoction on Col-IV, HA serum contents in rats with experimental alcoholic hepatic fibrosis. Methods: First, the model of experimental alcoholic liver fibrosis for this research was induced by intragastric administration of alcohol and lard, and it is divided into four groups: Group I with Chaihu Jiedu decoction intervention (inter- vention Group I), Group II with Chaihu Jiedu decoction and alcohol detoxification fat diet (intervention Group II), alcohol and high fat ingredients group (Model Group) and Blank Control Group. Researchers tested the serum from the four groups of rats which have been given lavage. At last, after weighing the weight of rats, researchers measured the contents of hyaluronic acid (HA) in type IV collagen (Col-IV) and type IV collagen (Col-IV) in hyaluronic acid (HA) by ELISA Kit. Results: After 12 weeks, the rats in each group were grayed, disorderly, thin and the stool was thin. Serum Col-IV, HA compared with the control group were significantly increased (P＜0.01）, Compared with Model Group, Serum Col-IV, HA in intervention Group I and II were significantly decreased (P＜0.01). The con- tents of HA in intervention Group I and Group II, Model Group and Blank Control group were 219.17±41.64, 184.89±22.83, 249.32±34.60, 123.48±25.85, and Col-IV contents were 9.59±1.81, 12.57±1.41, 19.71±6.04, 7.62±5.36. Conclusion: The research finds out that Chaihu Jiedu decoction may inhibit the expression of Col-IV and HA, which may have a certain effect on rat model of experimental alcoholic liver fibrosis. |
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