| 连晓英,白雪源,赵 静,张英杰,曹 静,王媛媛.多囊肾病小型猪肾脏组织中增殖和凋亡的改变[J].现代生物医学进展英文版,2017,17(11):2007-2011. |
| 多囊肾病小型猪肾脏组织中增殖和凋亡的改变 |
| Changes of Proliferation and Apoptosis in the Kidney of Mini-Pigs with Autosomal Dominant Polycystic Kidney Disease |
| Received:October 19, 2016 Revised:November 08, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.11.002 |
| 中文关键词: 常染色体显性多囊肾病 增殖 凋亡 小型猪 |
| 英文关键词: Autosomal Dominant Polycystic Kidney Disease Proliferation Apoptosis Mini-pig |
| 基金项目:国家重点研发计划项目（2016YFA0101002）；国家自然科学基金项目（81570659）；海南省社会发展科技专项资金项目（SF201341） |
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| 中文摘要: |
| 摘要 目的：观察ADPKD小型猪肾脏组织中细胞增殖和凋亡的改变。方法：使用PKD1基因敲除的小型猪模型，用Western blot和免疫组化方法检测肾脏组织中细胞增殖指标PCNA；增殖相关的mTOR信号通路分子(phospho-mTOR、phospho-p70S6、phospho-4EBP1)和ERK信号通路分子(phospho-PKA、phospho-MEK、phospho-ERK)的表达水平以及内皮细胞粘附分子CD31以及凋亡相关蛋白Bcl-2、Bax、caspase 3的表达变化。结果：在ADPKD小型猪肾脏组织中，增殖指标PCNA表达显著升高。mTOR信号通路分子phospho-mTOR、phospho-p70S6、phospho-4EBP1水平明显升高，ERK信号通路分子中phospho-PKA、phospho-MEK、phospho-ERK水平明显升高。CD31表达明显升高，Bax/Bcl-2的比值以及caspase 3的表达水平显著升高。结论：本研究显示在ADPKD小型猪肾脏组织中，细胞增殖和凋亡信号通路明显激活。 |
| 英文摘要: |
| ABSTRACT Objective: To observe proliferation and apoptosis of the cell in the kidney of Mini-Pigs with ADPKD. Methods: We observed the protein expression changes of cell proliferation marker PCNA, proliferation-related mTOR signal pathway key molecules (phospho-mTOR, phospho-p70S6, phospho-4EBP1), ERK signal pathway key molecules (phospho-PKA, phospho-MEK, phospho-ERK), endothelial cell adhesion molecule-1（CD31）, Bax, Bcl-2 and caspase 3 in the kidney tissues of PKD1 deletion mini-pig model by West-ern blot and Immunohistochemical staining. Results: The results showed that the level of PCNA was markedly up-regulated. The molecules in mTOR signal pathway (phospho-mTOR, phospho-p70S6, phospho-4EBP1), ERK signal pathway (phospho-PKA, phos- pho-MEK, phospho-ERK) and CD31were markedly increased in ADPKD mini-pig kidney tissues. In addition, the Bax/Bcl-2ratio and the caspase3 were increased significantly. Conclusion: The research shown that the signal pathway of proliferation and apoptosis was activated in ADPKD mini-pig kidney tissues. |
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