| 孙浩明,张广发,姚 文,杨 栋,孙文靖.重组中华仓鼠卵巢细胞培养过程控制的研究进展[J].现代生物医学进展英文版,2017,17(10):1997-2000. |
| 重组中华仓鼠卵巢细胞培养过程控制的研究进展 |
| Control of Training Process in Recombinant Chinese Hamster Ovary Cells |
| Received:May 10, 2015 Revised:May 31, 2015 |
| DOI：10.13241/j.cnki.pmb.2017.10.052 |
| 中文关键词: 中华仓鼠卵巢细胞 蛋白表达 培养条件 优化条件 |
| 英文关键词: Chinese hamster ovary cells Protein expression Culturing conditions Optimization |
| 基金项目:国家自然科学基金项目（31271347） |
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| 中文摘要: |
| 摘要：中华仓鼠卵巢细胞（Chinese Hamster Ovary Cells，CHO）是科研及生产中用于蛋白表达的常用体系。与大肠杆菌相比，CHO获得高表达的细胞株所需时间更长，蛋白产量更低。因此，大规模培养细胞所需的成本较高，培养条件也不易掌握。但该体系产生的蛋白纯度高，因而被广泛用于工业生产中。本文对CHO细胞的培养方式、pH值测定、渗透压、溶氧及培养液成分的选择等多方面条件进行综述，为优化中华仓鼠卵巢细胞培养的策略及具体方法提供理论依据。 |
| 英文摘要: |
| ABSTRACT: Chinese hamster ovary cells(CHO) are the system for protein expression which have been commonly used in the scientific researches and productions. Compared with the E. coli, the CHO time required to obtain a high expression of cell line is longer, and the protein productionis lower. When it goes to large-scale cell culture, not only doseit needa high cost but also bemore difficult to grasp the conditions. However, this system can produce higher purity protein, whichis widely applied in the industrial production. In this paper, the important issues of CHO cell culture process are to be reviewed. Optimization strategies and specific methods such as training methods, pH, osmolality, dissolved oxygen and broth ingredients are to be discussed. |
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