| 杜盛超,张 玮,胡浩然,孙 源,赵必增.一种改良大鼠慢性脊髓压迫模型的建立与评价[J].现代生物医学进展英文版,2017,17(10):1806-1809. |
| 一种改良大鼠慢性脊髓压迫模型的建立与评价 |
| Establishment and Evaluation of a Modified Rat Model of Chronic Spinal Cord Compression |
| Received:November 03, 2016 Revised:November 26, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.10.002 |
| 中文关键词: 慢性脊髓压迫 动物模型 聚乙烯水凝胶 |
| 英文关键词: Chronic spinal cord compression Animal model Polyethylene hydrogel |
| 基金项目:上海市科委博士后科研基金项目（14R21411100） |
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| 中文摘要: |
| 摘要 目的：在已有脊髓压迫建模方式基础上，建立一种更好模拟慢性发病的颈脊髓压迫动物模型，并对其神经功能进行初步评价。方法：将20只SD大鼠随机分为模型组、对照组。采用聚氨酯薄膜包裹吸水膨胀性材料聚乙烯醇构建改良慢性膨胀物大鼠脊髓损伤模型，观察不同时间点大鼠行为学运动功能（Basso, Beattie&Bresnahan locomotor rating scale, BBB scale）评分；苏木精-伊红（hematoxylin-eosin，HE）染色观察脊髓组织形态变化；脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TdT-mediated dUTP nick end labeling，TUNEL）检测损伤段脊髓组织细胞凋亡情况。结果：手术后模型组大鼠的BBB评分逐渐降低，模型组大鼠各时间点BBB评分均明显低于对照组，且两组差异具有统计学意义(P<0.05)。HE染色见模型组大鼠较对照组有明显的组织损伤反应。TUNEL染色显示模型组大鼠凋亡细胞明显增多。结论：本研究成功制备了稳定可靠、操作简单的一种改良慢性脊髓压迫模型。 |
| 英文摘要: |
| ABSTRACT Objective: To establish a better simulation of the chronic disease animal model of cervical spinal cord compression on the basis of the existing spinal cord compression model, and to evaluate its neurological function. Methods: Twenty SD rats were randomly divided into two groups: control group and model group. A water swelling material polyvinyl alcohol with the size of 3.0 mm*1.5mm*0.7mm was wrapped with polyurethane film, which expanded over time to induce chronic compression in the spinal cord. In the model groups, the expanding compression material was inserted between the C5 and C6 laminae and dura, resulting in chronic spinal cord compressing. The same surgery were done in the control group except for the implanting of the water-swellable compression material. Basso Beattie Bresnahan (BBB) locomotor rating scale was used at different time points to evaluate rats' hind limb motor function. Hematoxylin-eosin (HE) staining was used to observe spinal cord tissue morphology. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay was used to test the spinal cord injury tissue apoptosis. Results: BBB score of the model group decreased gradually after the operation, and was significantly lower than that of the control operation group at each time point， the differences between the two groups were statistically significant(P<0.05). HE staining indicated that model group rats had significant tissue response to injury compared with control group. Pathological examination of the model group revealed spinal cord damage with the formation of cavity and scar. TUNEL staining showed there were many apoptotic cells in the model group, while no apoptotic cells were found in the control operation groups. Conclusion: The chronic spinal cord injury model established in this study has good reproducibility and stability, can be used in the study of chronic spinal cord injury. |
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