| 郑念举,郭文娴,叶俊丽,季婵婵,宋爱琴,宋 亮,孙立荣.CHFR, PARP-1基因对B细胞淋巴瘤Raji细胞增殖和凋亡的影响[J].现代生物医学进展英文版,2017,17(8):1430-1436. |
| CHFR, PARP-1基因对B细胞淋巴瘤Raji细胞增殖和凋亡的影响 |
| Effect of CHFR and PARP-1 on the Proliferation and Apoptosis of B-cell non-Hodgkin's Lymphoma Raji Cells |
| Received:September 27, 2016 Revised:October 22, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.08.007 |
| 中文关键词: CHFR基因 有丝分裂检测点 PARP-1基因 B细胞非霍奇金淋巴瘤 Raji细胞 |
| 英文关键词: CHFR mitotic checkpoint PARP-1 B-cell non-Hodgkin lymphoma Raji cell |
| 基金项目:山东省自然科学基金项目(Y2008c170,Q2008C04)；山东省优秀中青年科学家科研奖励基金项目( BS2012YY004)；青岛市自然科学基金项目(2012-1-3-2-(5)-nsh) |
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| 中文摘要: |
| 摘要 目的：探讨CHFR与聚(ADP-核糖)聚合酶1(PARP-1)基因对B细胞淋巴瘤Raji细胞增殖和凋亡的影响。方法：用5-Aza-dC处理Raji细胞，后通过qRT-PCR检测CHFR的mRNA表达水平，western blot评估CHFR、PARP-1的蛋白表达。经CHFR慢病毒转染Raji细胞后，用qRT-PCR和western blot评估RAJI细胞中的CHFR、PARP-1变化。通过CCK-8法测定细胞的增殖情况，流式细胞术检测细胞周期的变化。结果：与对照组相比，经5-Aza-dC处理 Raji组的CHFR mRNA表达显著上调(P <0.01)，PARP-1 mRNA水平和蛋白表达水平明显降低(P <0.05)。与对照组相比，ShRNA组CHFR的mRNA表达显着下调(均P <0.01)，PARP-1 mRNA和蛋白表达水平升高(P <0.05)。CCK结果显示CHFR ShRNA组细胞活力明显低于对照组(P <0.05)。流式细胞术结果显示CHFR沉默后细胞凋亡率降低(P <0.05)。结论：CHFR可能通过PARP-1调控B淋巴细胞的增殖和凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of checkpoint with forkhead-associated (FHA) and RING finger domains (CHFR) with poly(ADP-ribose) polymerase 1 (PARP-1) on the proliferation and apoptosis of B-cell non-Hodgkin's lymphoma Raji cells. Methods: Quantitative real-time PCR (qRT-PCR) and western blot were used to detect the CHFR, PARP-1 mRNA and protein expressions in Raji cells treated by 5-Aza-dC and transfected by CHFR lentivirus. Cell proliferation was analyzed by a standard Cell Counting Kit-8 (CCK-8) assay, and the cell cycle changes were detected by flow cytometry. Results: The mRNA expression of CHFR in the 5-Aza-dC Raji group was significantly up-regulated compared with that in the control group (P < 0.01). PARP-1 levels were decreased in mRNA levels and the protein expression levels compared with control group(all P<0.05). The mRNA expression of CHFR in the ShRNA group was significantly down-regulated compared with that in the control and normal group (both P < 0.01). The mRNA and protein expression levels of PARP-1 were increased in ShRNA group compared with the control group and negative control group(P < 0.05). The CCK results suggested that the decrease in CHFR expression promoted the cell proliferation(P<0.05). And Flow cytometry results demonstrated that the cell apoptosis rate was decreased after CHFR silencing (P<0.05). Conclusion: These data suggested that CHFR might regulate the proliferation and apoptosis of B-cell non-Hodgkin's lymphoma Raji cells through PARP-1. |
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