| 秦娟 晏伟 王三喜 王海燕 唐晓梅 龙兆博.采用不同方法制作胸腹水细胞块的效果比较及临床价值研究[J].现代生物医学进展英文版,2017,17(7):1357-1359. |
| 采用不同方法制作胸腹水细胞块的效果比较及临床价值研究 |
| Clinical Value of Different Methods in Preparation of Pleural and PeritonealEffusion Cell Blocks |
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| DOI： |
| 中文关键词: 胸腔积液 腹水 细胞块 临床价值 |
| 英文关键词: Pleural effusion Peritoneal effusion Cell blocks Clinical value |
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| 中文摘要: |
| 目的：比较不同方法制作胸腹水细胞块的效果以优化胸腹水细胞块制作程序，并对其临床价值进行探讨。方法：以2014 年3
月至2015 年3 月我科收集的胸腹水标本120 例为研究对象，将每样标本平均分成三组，使用三种不同方法(试管包埋法、直接离
心法、细胞块试剂盒法)制作胸腹水细胞块。对不同方法制作细胞块的成功率、完整性及细胞切片恶性细胞的检出率进行考察与比
较。结果：细胞块试剂盒法成功率最高，为96.67 %，试管包埋法次之，成功率为92.50 %，二者相比无显著差异（P>0.05）。直接离心
法制作成功率为80.83 %，远远低于试管包埋法及细胞块试剂盒法，差异有统计学意义（P<0.05）。细胞块试剂盒法制作的细胞块完
整性最高，完整标本所占比例为96.67 %，试管包埋法次之，完整率为94.17 %，二者相比无显著差异（P>0.05）。直接离心法制作完
整率为68.33 %，远远低于试管包埋法及细胞块试剂盒法，差异有统计学意义（P<0.05）。三种方法恶性细胞检出率以细胞块试剂盒
最高，与其他两种方法比较差异有统计学意义（P<0.05）。结论：细胞块试剂盒法制作胸腹水细胞块具有最高的成功率及完整性，并
且可以显著提高恶性细胞检出率，值得广泛使用。 |
| 英文摘要: |
| Objective:To compare the efficacy of different methods in preparation of pleural and peritoneal effusion cell blocks in
order to optimize the procedure, and explore the clinical efficacy.Methods:120 cases pleural and peritoneal effusion selected in our department
from March 2014 to March 2015 were divided into 3 groups using different methods (tube embedding method, centrifugal
method and cell blocks kit method) in preparation of cell blocks. The success rate, completeness and detection rate of malignant cells of 3
groups were observed and compared.Results:The success rate of cell blocks kit (96.67 %) was the highest, then the tube embedding
method (92.50 %), which had no statistically difference (P>0.05). The success rate of direct centrifugal method was obviously lower than
that of the other two methods with statistically significance (P<0.05). The completeness of cell blocks kit was the highest with the rate of
96.67 %, then the tube embedding method (94.17 %), which had no statistically difference (P>0.05). The completeness rate of direct centrifugal
method was 68.33 %, which was obviously lower than that of the others with statistically difference (P<0.05). The detection rate
of malignant cells of cell blocks kit was higher than that of the others with statistically significance (P<0.05).Conclusion:Using cell
blocks kit in preparation of pleural and peritoneal effusion can receive the best success and completeness rate, and contribute to improve
the detection rate of malignant cells, worthy of application. |
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