| 于文华 张孟玲 郭菲菲 孙向荣 徐珞.侧脑室注射orexin-A对大鼠昼夜摄食的影响[J].现代生物医学进展英文版,2017,17(7):1233-1236. |
| 侧脑室注射orexin-A对大鼠昼夜摄食的影响 |
| Effect of Orexin-A Administered ICV on the Nycterohemeral Food Intake ofRats |
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| DOI： |
| 中文关键词: orexin-A 摄食 昼夜节律 |
| 英文关键词: Orexin-A Food intake Circadian rhythms |
| 基金项目:国家自然科学基金项目（81470815，81270460，81300281，81500414）；
山东省优秀中青年科学家科研奖励基金项目（BS2014YY009）；青岛市科技局基金项目（13-1-4-170-jch；14-2-3-3-nsh） |
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| 中文摘要: |
| 目的：探讨侧脑室注射orexin-A 对大鼠昼夜摄食的影响。方法：将Wistar 大鼠随机分组，采用单剂量侧脑室注射和连续侧脑
室注射以及外周注射法，分别于日间和夜间给药，测量大鼠24 小时内各阶段的摄食量以及相应生化指标。结果：在光照期间，侧
脑室微量注射orexin-A，大鼠4 小时内摄食量显著增加(P<0.05)，且呈剂量依赖关系(P＜0.05)。在夜间初期(18:00)侧脑室注射
orexin-A，大鼠食物摄入量无显著差异(P＞0.05)。但在中午12:00给予侧脑室注射orexin-A，注射后4 小时内大鼠摄食量显著高于
NS对照组(P＜0.05)。连续8 日给予orexin-A 侧脑室注射，可使注射后日间摄食量显著增加(P＜0.05)，而夜间摄食量显著减少(P＜
0.05)，但24小时内总的摄食量不变(P＞0.05)。orexin-A并未改变棕色脂肪组织温度、末梢血糖、血浆瘦素等指标的水平。结论：
orexin-A 对大鼠摄食的调节具有昼夜节律性。 |
| 英文摘要: |
| Objective:To investigate the effect of the orexin-A on nycterohemeral food intake in rats.Methods:Male wistar rats
were randomly assigned to accept a single intracerebroventricul-ar (ICV) injection, continuous ICV injection and perineural injection,
dosed in the daytime and dark phase, then measured the food intake of rats during the whole day of various phases and corresponding
biochemical indexes.Results:Compared with NS group, inject orexin-A into ICV in the light phase (08:00) dose-dependently increased
food intake following the next 4 h in rats (P<0.05). Food consumption of rats was unchanged after a single administered ICV with orexin-
A in the early dark times (18:00) (P＞0.05). Compared with NS group, administered orexin-A into ICV in the light times (12:00) increased
food consumption following the next 4 h in rats (P<0.05). Continuous administered ICV with orexin-A significantly increased the
food intakes during the light phase (P＜0.05), and reduced it during the dark phase (P＜0.05), but total 24 h food intake was moving (P＞
0.05). The level of the temperature of brown adipose tissue, peripheral blood glucose, plasma leptin and other indexes were not changed
after ICV infusion of orexin-A.Conclusion:The regulation of Orexin-A on food intake in rats was circadian rhythm. |
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