| 罗勇 潘俊 程意 高晶晶 韩锋锋.不同烟熏强度大鼠伸趾长肌抗氧化能力和氧化应激损伤[J].现代生物医学进展英文版,2017,17(7):1206-1209. |
| 不同烟熏强度大鼠伸趾长肌抗氧化能力和氧化应激损伤 |
| Anti-oxidants and Oxygen Stress Damage of Skeletal Muscle in Rats withDifferent Intensities of Passive Cigarette Smoking |
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| DOI： |
| 中文关键词: 烟熏 大鼠 骨骼肌 抗氧化 氧化应激 |
| 英文关键词: Smoking Rats Skeletal muscle Anti-oxidants Oxygen stress |
| 基金项目:上海市卫生和计划生育委员会资助课题（20134141） |
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| 中文摘要: |
| 目的：研究不同烟熏强度对SD 大鼠骨骼肌组织抗氧化能力及氧化应激损伤的影响，探讨COPD骨骼肌功能障碍的机制。方
法：40 只SD大鼠随机分为对照组、实验1 组、实验2 组、实验3 组，每组各10 只。实验1 组、实验2 组、实验3 组分别给予4 周、
12 周、20 周的烟熏强度，烟熏结束后取大鼠双侧伸趾长肌，用酶联免疫吸附测定法（ELISA）方法检测各组大鼠的伸趾长肌组织匀
浆谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和丙二醛
（MDA）的浓度，伸趾长肌组织蛋白质提取液羰基化蛋白（PC）的浓度、伸趾长肌组织DNA 中8- 羟基脱氧鸟苷（8-OHdG)的含量。
结果：与对照组相比，实验1 组、实验2 组、实验3 组GSH、GSH/ GSSG、SOD显著降低（P＜0.05～P＜0.01），GSSG 显著升高（P＜
0.05），实验1 组GPx显著降低（P＜0.05），实验1 组CAT显著升高（P＜0.05），实验2 组、实验3 组MDA、8-OHdG 显著增高（P＜
0.05～P＜0.01），实验3 组PC 显著增高（P＜0.05）。结论：烟熏可使大鼠骨骼肌组织抗氧化/氧化失衡，导致氧化应激损伤，并与烟
熏强度相关。 |
| 英文摘要: |
| Objective:To study the effect of anti-oxidants and oxygen stress damage on skeletal muscle in rats with different intensities
of passive cigarette smoking and to explore mechanisms of skeletal muscle dysfunction in COPD.Methods:Forty rats were divided
into 4 groups randomly: control group, experiment 1 group, trial 2 and 3 group. Rats of trial 1, 2, 3 groups were exposed passive cigarette
smoking for 4 weeks, 12 weeks and 20 weeks respectively. GSH, GSSG, GPx, CAT, SOD and MDA, PC, 8-OHdG in the extensor digitorum
longus of rats were detected through ELISA.Results:The expression levels of GSH, GSH/GSSG, SOD decreased and GSSG increased
significantly in all trial groups (P＜0.05～P＜0.01) , GPx decreased and CAT increased significantly in trial 1 group (P＜0.05) ,
and MDA, 8-OHdG in trial 2, 3 groups and PC in trial 3 group increased significantly in comparison with control group (P＜0.05～P＜
0.01).Conclusion:Cigarette smoking can cause imbalance of anti-oxidants /oxygen stress and results in oxygen stress damage in the
distal skeletal muscle tissue. |
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