| 陶亮 唐海利 张伟东 李燕 熊利泽.敲除NDRG2 基因的AD模型小鼠的构建与鉴定[J].现代生物医学进展英文版,2017,17(6):1020-1023. |
| 敲除NDRG2 基因的AD模型小鼠的构建与鉴定 |
| N-myc DownstreamRegulated Gene 2 Conventional Knockout AD Mice:Breeding and Genetic Identification |
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| DOI： |
| 中文关键词: NDRG2 基因敲除 阿尔茨海默症 |
| 英文关键词: N-myc downstreamregulated gene 2 Gene knockout Alzheimer's disease |
| 基金项目:国家自然科学基金面上项目（81471110） |
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| 中文摘要: |
| 目的：构建与鉴定NDRG2 基因全身敲除的阿尔茨海默症（AD）小鼠模型。方法：将NDRG2-/-、APP/PS1 进行饲养杂交繁殖，
将通过PCR 技术鉴定基因型为NDRG2+/-APP/PS1 的子一代小鼠再与NDRG2-/- 小鼠回交获得子二代小鼠，提取子二代小鼠的
基因组DNA，再利用PCR 方法扩增NDRG2和APP/PS1 基因片段，并进行琼脂糖凝胶电泳检测，获得4 种基因型的小鼠。结果：
选取基因型为NDRG2-/-APP/PS1 的小鼠即为全身NDRG2 敲除且淀粉样蛋白基因过表达的阿尔茨海默症模型小鼠。应用PCR
方法鉴定NDRG2 基因全身敲除的AD小鼠模型。成功获得NDRG2基因全身敲除的AD 小鼠，该基因型小鼠有繁殖能力，其繁
殖符合孟德尔遗传规律。结论：成功构建NDRG2 基因敲除的阿尔茨海默症模型小鼠，为进一步研究NDRG2基因在阿尔茨海默
症病理发展过程中的作用机制及新的治疗方法的研究提供模型基础。 |
| 英文摘要: |
| Objective:To breed N-myc downstream regulated gene 2 (NDRG2) conventional knockout AD mice.Methods:Two
mouse models, namely NDRG2-/- and APP/PS1 were interbred, and the first-generation offsprings that through the use of PCR with the
NDRG2+/-APP/PS1 genotype were backcrossed with NDRG2-/- to obtain the second-generation mice. Genomic DNA was extracted
fromthe second-generation mice for PCR to amplify the NDRG2 and APP/PS1 gene fragments followed by agarose gel electrophoresis to
verify their sizes and we obtained four different genotypes of mice.Results:The mice with the NDRG2-/-APP/PS1 genotype were
selected as the NDRG2 conventional knockout AD mice.Tissue samples were collected from the mice for the expression of NDRG2 or
APP/PS1 using PCR.Conclusion:Genotyping results showed APP/PS1 was expressing in the NDRG2 conventional knockout mice,
suggesting successful establishment of NDRG2 conventional knockout AD mice. The mice we obtained were fertile, and their breeding
pattern followed the laws of Mendelian inheritance. More important，it provides a model foundation for further study of gene NDRG2 in
AD-related pathology and new therapy. |
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