| 张春强,张 友,张 樱,陈 琼,尹龙武.CCL4在病毒性心肌炎中的作用及其机制研究[J].现代生物医学进展英文版,2017,17(4):636-639. |
| CCL4在病毒性心肌炎中的作用及其机制研究 |
| Research on the Function and Mechanism of CCL4 in Viral Myocarditis |
| Received:June 14, 2016 Revised:June 30, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.04.009 |
| 中文关键词: CCL4 病毒性心肌炎 IL-1β TNF-α |
| 英文关键词: CCL4 Viral myocarditis Interleukin-1β Tumor necrosis fator-α |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的：观察巨噬细胞炎性蛋白-1β(MIP-1β/CCL4)在病毒性心肌炎小鼠血清以及心肌组织中的变化，初步探讨CCL4在病毒性心肌炎中发挥的作用。方法：雄性Balb/c小鼠随机分成对照组15只和病毒性心肌炎组25只，病毒性心肌炎组腹腔注射病毒液，对照组腹腔注射Eagle's培养液。15 d处死小鼠，通过HE染色观察小鼠心肌病理的变化；RT-PCR观察CCL4以及促炎因子的mRNA水平，Western blotting观察CCL4蛋白水平的变化。结果：与正常对照组比，病毒性心肌炎组小鼠生存质量降低，生存率也显著降低；病毒性心肌炎组小鼠心肌炎性浸润严重，心肌组织中的CCL4、IL-1β、TNF-α mRNA水平显著增高，CCL4蛋白水平也明显增高，并且CCL4的浓度和蛋白表达水平与心肌病变积分呈正相关。结论：病毒性心肌炎CCL4的表达显著增加，并且与心肌病变程度相关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the expression of CCL4 in viral myocarditis and to investigate the mechanism. Methods: Male Balb/c mice were randomly divided into control group of 15 cases and viral myocarditis group of 25 cases, viral myocarditis group was injected intraperitoneally, and the control group was injected with Eagle's culture solution. 15d mice were sacrificed and the pathological changes of myocardium were observed by HE staining. CCL4 and mRNA levels of pro-inflammatory factors were observed through RT-PCR, and changes of CCL4 protein levels were observed by blotting Western. Results: Compared with the normal control group, the survival rate of mice with viral myocarditis was decreased, and the survival rate was decreased significantly.severe infiltration of myocarditis in mice with viral myocarditis, and the levels of CCL4, IL-1, TNF-α, mRNA and were significantly increased, and the level of CCL4 protein was significantly increased, and the concentration of CCL4 and protein expression were positively correlated with the score of myocardial lesion. Conclusion: The expression of CCL4 is significantly increased in viral myocarditis, and correlated with the degree of myocardial lesion. |
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