| 劳金泉,邓 燕,张 锋,彭小飞,叶冰冰,庞玉生.经胸右心室穿刺法测量大鼠右心室压力的研究[J].现代生物医学进展英文版,2017,17(4):619-623. |
| 经胸右心室穿刺法测量大鼠右心室压力的研究 |
| Research of Measurement of Right Ventricle Pressure by Right Ventricle Punctured through Chest in Rats |
| Received:April 16, 2016 Revised:May 14, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.04.005 |
| 中文关键词: 大鼠 右心室 肺动脉 压力 |
| 英文关键词: Rat Right ventricle Pulmonary artery Pressure |
| 基金项目:国家自然科学基金项目（81160040） |
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| 中文摘要: |
| 摘要 目的：探索一种技术简单、操作方便、重复性好、容易掌握的大鼠右心室压力测量方法。方法：取健康Sprague Dawley大鼠20只，随机分为实验组（n=10），对照组（n=10）。实验组使用一次性静脉输液针（0.45×13.5 mm）经胸直接穿刺右心室测量右心室压力，对照组使用右心导管，经颈外静脉沿右心房插入右心室测量右心室压力。比较两者操作时间、成功率、右心室压力等指标。结果：实验组从麻醉开始到成功测出右心室压力的时间5.10±1.32 min，对照组为25.21±10.30 min（P＜0.05）。两种方法所检测到的右心室收缩压、右心室舒张压、右心室平均压无统计学差异（P＞0.05）。实验组大鼠成功率100 %，对照组60 %（P＜0.05）。结论：经胸右心室穿刺法能准确、快速穿刺到右心室并能准确测量右心室压力。 |
| 英文摘要: |
| ABSTRACT Objective: To explore a technique for measuring right ventricular pressure with simple technique, convenient operation, good repeatability and easy to master. Methods: Twenty healthy Sprague Dawley rats were divided into the experimental group (n=10) and the control group (n=10) randomly. The disposable venous infusion needle (0.45×13.5 mm) was punctured into the right ventricle directly through chest to measure the right ventricular pressure of rats in the experimental group. For the rats in control group, the right ventricular polyethylene catheter was inserted into right ventricular through right external jugular vein and right atrium so as to measure right ventricular pressure. The operation time, right ventricle pressure and successful rate for measuring were calculated in the two groups. Results: No significant differences of right ventricle systolic pressure, right ventricle diastolic pressure, and right ventricle mean pressure were found between the experimental and control group (P＞0.05). The time spent from anesthetization to measuring was 5.10±1.32 min in the experimental group while 25.21±10.30 min in the control group, and the difference between two groups was statistical significant (P<0.05). The percentage of successful measurement was 100 % in the experimental group and 60 % in the control group with statistical significance (P＜0.05). Conclusion: The right ventricular pressure could be measured accurately and rapidly by the technique of right ventricle punctured through chest in rats. |
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