张怡 陈思 谢晓丹 张蕾 张倩.黑龙江地区400 例乙型肝炎病毒基因分型与耐药突变研究[J].现代生物医学进展英文版,2017,17(3):485-488. |
黑龙江地区400 例乙型肝炎病毒基因分型与耐药突变研究 |
Research of Hepatitis B virus Genotyping and Variation of Drug Resistancein 400 Cases in Heilongjiang Area |
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DOI: |
中文关键词: 乙型肝炎 基因芯片 基因分型 耐药突变 |
英文关键词: Hepatitis B Gene chip Genotype Drug-resistance mutation |
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中文摘要: |
目的:运用基因芯片技术分析黑龙江地区乙型肝炎病毒(HBV)基因型分布特征及基因耐药变异情况。方法:随机选择2012
年11 月至2015 年11 月本医院乙型肝炎患者血清样本400例,应用PCR- 反向点杂交的基因芯片技术对样本血清中HBV基因
型及常见4 类抗病毒药物耐药相关的多个位点进行检测,并进行数据分析。结果:400 例样本中基因型分布以C 型为主占83.25
%(333 例),B 型7.25 %(29 例)、D 型0.25 %(1例)及混合基因型2.75 %(11 例);耐药突变位点检出188 例,总耐药率为45.19
%,其中突变位点236T(4.61 %)提示阿德福韦酯单项耐药,耐药率为5.82 %(10 例),与拉米夫定耐药相关的为126 例,突变位点
以rt204I和(rt180M+rt240V)为主,显著高于其他抗病毒类药物,耐药风险较高。结论:黑龙江地区乙型肝炎基因分型以C 为主,B
型和其它混合型较少,且更容易对拉米夫定产生耐药。 |
英文摘要: |
Objective:To investigate the distribution characteristics of hepatitis B virus (HBV) genotypes and the variation situation
of drug resistance gene in Heilongjiang area by the gene chip technology.Methods:Serum samples were randomly collected from
400 patients of hepatitis B in our hospital from November 2012 to November 2015. The HBV genotypes and drug resistance associated
multiple sites in common 4 types of antiviral drugwere detected by the gene chip technology of PCRcombinedwith reverse dot blot.Results:Among 400 samples in Heilongjiang area, 83.25 %genotypes were genotype C (333 cases), type B 7.25 % (29 cases), type D 0.25 % (1
case) and mixed type 2.75%(11 cases); 188 sites were found to be resistance mutants, the total resistance frequency was 45.19 %, rt236T
mutations in 10 samples prompted drug resistance-mutations for Adefovir drug, and the resistance frequency was 5.82 %, and 126 samples
drugs resistance-mutations was Lamivudine drug in major of rt204I and (rt180M+rt204V). 126 cases were related with Lamivudine
resistance, which was significantly higher than other antiviral drugs, the risk of drug resistance was greater.Conclusion:The main HBV
genotype is the genotype C in Heilongjiang area, genotype B and mixed genotype are unusual, which is more likely to happen resistance
to Lamivudine. |
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