| 浦清 华玮 居培红 戴晓勇 周季兰1 李铮.白细胞介素-6 在急性胰腺炎中的作用及其机制研究[J].现代生物医学进展英文版,2017,17(2):308-312. |
| 白细胞介素-6 在急性胰腺炎中的作用及其机制研究 |
| The Interleukin-6 is Involved in Acute Pancreatic in Mice and ItsMechanisms |
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| DOI： |
| 中文关键词: 白细胞介素-6 急性胰腺炎 细胞外调节细胞激酶 |
| 英文关键词: Interleukin-6 Acute pancreatic ERK |
| 基金项目:上海市自然科学基金项目（13ZR1318932） |
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| 中文摘要: |
| 目的：明确白细胞介素-6( IL-6)在小鼠急性胰腺炎中的作用及其机制研究。方法：通过胰胆管结扎的方法诱导小鼠急性胰腺
炎；分离小鼠胰腺腺泡细胞。采用ELISA 方法检测胰腺组织或腺泡细胞裂解物中的细胞因子；通过western blot 分析检测组织或
细胞中IL-6或ERK 表达。结果：IL-6 浓度在胰腺组织和腺泡细胞中显著增加(P<0.05)。在离体原代小鼠腺泡细胞，TNF-alpha刺激增
加IL-6 释放(P<0.05)；与此同时，IL-6 刺激可增加其它促炎性细胞因子的释放，两者都涉及ERK MAP 激酶通路。黄酮类化合物木
犀草素抑制IL-6 刺激引起白细胞介素-6 (IL-6)和人巨嗜细胞激活蛋白-1(CCL2/MCP-1)释放。最后进一步证实，IL-6 激活人胰腺
组织中的ERK。结论：IL-6 在急性胰腺炎中增加，激活炎症通路并加重急性胰腺炎。 |
| 英文摘要: |
| Objective:To investigate whether interleukin-6 (IL-6) exacerbates acute pancreatic inflammation in mice and its
mechanisms.Methods:The pancreatic acinar cells were isolated from mice with duct ligation-induced acute pancreatitis. ELISA was
used to measure cytokines in pancreas tissue or acinar cells lysates, and western blot analysis was applied to detect the expression of IL-6
or ERK.Results:IL-6 concentration was increased in the pancreas and acinar cell (P<0.05). In isolated mouse pancreatic acinar cells,
TNF-alpha stimulation increased IL-6 release while IL-6 stimulation increased release of proinflammatory cytokine, and both involved the
ERK MAP kinase pathway. The flavonoid luteolin inhibited the release of IL-6-stimulated IL-6 and CCL2/MCP-1. Also, IL-6 activated
ERK in human pancreatic tissue.Conclusion:IL-6 is induced in acute pancreatitis, activates acinar cell proinflammatory pathways and
exacerbates acute pancreatic inflammation. |
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