| 宋凡倩 高宁宁 孙莉尧 曹志超 刘平 葛红岩.截短型rhtBIGH3-(RGD)2蛋白通过上调miR-126 表达抑制
人脐静脉内皮细胞的生物活性[J].现代生物医学进展英文版,2017,17(2):225-228. |
| 截短型rhtBIGH3-(RGD)2蛋白通过上调miR-126 表达抑制
人脐静脉内皮细胞的生物活性 |
| Biological Activities of HUVECS Is Inhibited by Truncated rhtBIGH3-(RGD)2 Protein though the Up-regulation of miR-126 |
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| DOI： |
| 中文关键词: rhtBIGH3-(RGD)2蛋白 miR-126 HUVEC 角膜新生血管 Caspase-3 |
| 英文关键词: RhtBIGH3-(RGD)2 protein MiR-126 HUVEC Corneal neovascularization Caspase-3 |
| 基金项目:黑龙江省教育厅面上项目（12541516）；国家自然科学青年基金项目（81300728） |
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| 中文摘要: |
| 目的：探讨miR-126在截短型rhtBIGH3-(RGD)2蛋白抑制HUVEC 细胞生物学活性中的作用。方法：体外培养VEGF孵化的
人脐静脉内皮细胞(VEGF-HUVEC)，分别加入rhtBIGH3-(RGD)2蛋白终浓度为0 和100 滋g/mL，作用24、48、72 h 条件下，分别检
测Caspase-3 活性和miR-126 表达水平。在rhtBIGH3- (RGD)2蛋白终浓度为0 和100 ug/mL 时，分别加入miR-126 mimic 和
miR-126 inhibitor，作用VEGF-HUVEC 48 h后，Real-time PCR检测miR-126 表达水平，通过检测Caspase-3 活性来检测细胞凋亡
情况。结果：在VEGF-HUVEC 中，当rhtBIGH3-(RGD)2蛋白终浓度为100 ug/mL条件下，Caspase-3 水平升高，miR-126 表达水平
升高，在48 h下达到最高峰。在VEGF-HUVEC 中，当rhtBIGH3-(RGD)2蛋白终浓度分别为100 ug/mL 条件下，加入miR-126
mimic 后，miR-126 表达升高，Caspase-3 水平升高; 加入miR-126 inhibitor 后，48 h后检测miR-126表达下降,Caspase-3 水平也下
降。结论：截短型rhtBIGH3-(RGD)2蛋白通过上调miR-126 表达从而促进细胞凋亡、抑制HUVEC 生物活性，从而抑制角膜新生血
管的发生 |
| 英文摘要: |
| Objective:To explore the effect of truncated rhtBIGH3 protein in inhibiting the biological activities of HUVECS.Methods:Human umbilical vein endothelial cells (HUVECS) were cultured with VEGF in vitro, supplemented with 0 ug/mL and 100
ug/mL rhtBIGH3- (RGD)2 protein. HUVECS were incubated for 24 h, 48 h, 72 h, and testing Caspase-3 activities and miR-126
expression level. Added miR-126 mimic and miR-126 inhibitor when the rhtBIGH3-(RGD)2 concentration was 0 ug/mL and 100 ug/mL,
after 48 h, use Real-time PCR testing the miR-126 expression level, and test the apoptosis though the activity of Caspase-3.Results:In
the VEGF-HUVEC supplemented with 100 ug/mL rhtBIGH3-(RGD)2 protein, the expression of both Caspase-3 and miR-126 raised, and
reach the peak at 48 h. In VEGF-HUVEC, when the final concentration of rhtBIGH3- (RGD)2 protein was 100 ug/mL, after the addition
of mimic miR-126, miR-126 and Caspase-3 expression increased, after adding inhibitor 48 h, miR-126 and Caspase-3 expression
decreased.Conclusion:The truncated rhtBIGH3- (RGD)2 protein promote apoptosis and inhibit HUVEC activity by increasing miR-126
expression, thus inhibiting corneal neovascularization. |
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