| 童 萌,原慧萍,张诗琦,赵 洋,谭欣慈.RegIV在视网膜母细胞瘤中的表达及其与血管新生的相关性分析[J].现代生物医学进展英文版,2017,17(1):88-92. |
| RegIV在视网膜母细胞瘤中的表达及其与血管新生的相关性分析 |
| Expressions of RegIV in Retinoblastoma and Its Correlation with Angiogenesis |
| Received:April 07, 2016 Revised:April 26, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.01.022 |
| 中文关键词: Reg IV 视网膜母细胞瘤 VEGF-A VEGF-C 血管新生 |
| 英文关键词: Reg IV Retinoblastoma VEGF-A VEGF-C Angiogenesis |
| 基金项目:四川省科技厅基金项目（17ZDYF2753） |
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| 中文摘要: |
| 摘要 目的：研究RegIV（再生基因Ⅳ）在视网膜母细胞瘤中的表达及其与血管新生的相关性。方法：选择2012年6月-2014年6月在我院行眼球摘除术治疗的视网膜母细胞瘤患儿16例为研究对象。采用免疫组化染色法对16例视网膜母细胞瘤患者肿瘤组织及16例正常视网膜组织标本中的RegIV、VEGF-A（血管内皮生长因子-A）、VEGF-C（血管内皮生长因子-C）的表达进行检测，并分析RegIV的表达与视网膜母细胞瘤患者的临床病理特征及VEGF-A、VEGF-C表达的相关性，同时比较RegⅣ阳性表达病例肿瘤组织与正常视网膜组织中微血管密度(MVD)之间的差异。结果：视网膜母细胞瘤组织中RegⅣ、VEGF-A、VEGF-C的表达水平均较正常视网膜组织显著上升，差异有统计学意义(P＜0.05)。视网膜母细胞瘤患者瘤组织中RegⅣ、VEGF-A、VEGF-C表达均与其临床分期、病理分型及视神经侵犯与否均显著相关(P<0.05)，与性别、发病部位无关(P＞0.05)。视网膜母细胞瘤组织中RegⅣ的表达与VEGF-A表达呈正相关(r=0.696，P＜0.05)，与VEGF-C表达呈正相关(r=0.728，P＜0.05)。视网膜母细胞瘤组织中RegⅣ阳性表达的病例MVD值为(59.67±1.43)，明显高于正常视网膜组织中MVD值(30.53±2.31)(P＜0.05)；视网膜母细胞瘤组织中RegⅣ阴性表达病例MVD值为（38.04±2.86），与正常视网膜组织中MVD值比较无明显差异（P＞0.05）。结论：RegIV在视网膜母细胞瘤中呈高表达，且与肿瘤的侵袭与转移相关，其可能通过促进VEGF-A、VEGF-C表达促进血管新生，进而加速视网膜母细胞瘤的发生和进展。 |
| 英文摘要: |
| ABSTRACT Objective: To study the expression of regenerating islet-derived family member 4 (RegIV) in retinoblastoma and its correlation with angiogenesis. Methods: 16 cases of patients with retinoblastoma who underwent operation in our hospital from June 2012 to June 2014 were selected as the research object. The positive expression rates of RegIV, VEGF-A and VEGF-C between retinoblastoma tissues and normal tissues were compared, the correlation of clinicopathological features with the expressions of RegIV, VEGF-A and VEGF-C were analyzed, and theh microvessel density (MVD) between RegIV positive expression tumor tissue and normal tissue were compared. Results: Compared with the normal tissues, the Reg IV, VEGF-A and VEGF-C expressions were higher in the retinoblastoma tissues(P<0.05). The Reg IV, VEGF-A, VEGF-C expressions were significantly correlated with the clinical stage, patho- logical type, optic nerve invasion retinoblastoma (P<0.05), but had nothing to do with gender, site of onset (P>0.05). Pearson correlation analysis showed the expression of Reg IV was positively correlated with VEGF-A(r=0.696, P＜0.05); the expression of Reg IV was posi- tively correlated with VEGF-C(r=0.728, P＜0.05). The MVD value of Reg IV positive expression was (59.67±1.43), which was signifi- cantly higher than that of the normal retinal tissues (30.53±2.31) (P<0.05); the MVD value of Reg IV negative expression tissues was(38.04±2.86), which had no difference compared with the normal retinal tissues(P＞0.05). Conclusion: The positive expression rates of Reg IV was highly expressed in the retinoblastoma tissue and correlated with the invasion and migration, it could accelerate the develop- ment of the retina through promoting the angiogenesis mediated by VEGF-A and VEGF-C. |
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