| 徐金锴,李宗禹,苏清华,赵 军,马建仓.自分泌运动因子在肝细胞癌侵袭及转移中的作用[J].现代生物医学进展英文版,2017,17(1):32-35. |
| 自分泌运动因子在肝细胞癌侵袭及转移中的作用 |
| The Effect of Autocrine Motility Factor on the Invasion and Metastasis of Hepatocellular Carcinoma |
| Received:August 08, 2016 Revised:August 29, 2016 |
| DOI：10.13241/j.cnki.pmb.2017.01.008 |
| 中文关键词: 肝细胞癌 AMF 转移 侵袭 |
| 英文关键词: Hepatocellular carcinoma Autocrine Motility Factor(AMF) Invasion Metastasis |
| 基金项目:陕西省科学技术厅基金项目（2014K11-03-03-16） |
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| 中文摘要: |
| 摘要 目的：探讨自分泌运动因子(AMF)在人肝细胞癌侵袭和转移中的作用。方法：人肝细胞系LO2和人肝细胞癌细胞株MHCC97-H作为实验材料，检测二者AMF的表达水平；设计并合成针对AMF基因序列的双链小干扰RNA转染高转移性人肝癌细胞株MHCC97-H，Western blot检测AMF基因的蛋白的表达水平；通过MTT实验检测转染后细胞的增殖力；通过体外Tran- swell小室对比沉默AMF基因前后的肝癌细胞的迁移力和侵袭力；最后用细胞悬液皮下接种小鼠，观察沉默AMF基因前后肝细胞的成瘤能力。结果：AMF在MHCC97-H的表达量较高；将双链小干扰RNA转入MHCC97-H后，AMF的表达显著降低(P＜0.05)；沉默AMF基因序列后，MHCC97-H的增殖力、迁移力和侵袭力均有明显下降(P＜0.05)；用细胞悬液皮下接种小鼠沉默AMF基因的MHCC97-H形成的肿瘤体积小于对照组(P＜0.05)。结论：AMF基因可调节肝癌细胞的迁移和侵袭。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of autocrine motility factor invasion and metastasis of hepatocellular carcinoma. Methods: Human liver LO2 cells and human liver cancer MHCC97-H cells were used as the research object, and the protein expression levels of AMF was tested. Chemically synthesized siRNA duplex targeting the AMF gene (AMF-siRNA) was transiently transfected into MHCC97-H cells protein expression levels of AMF was detected by Western blot; Cell proliferation was determined by MTT experiment tests after transfection. The effect of AMF knockdown on cell invasion and migration was analyzed by Transwell chamber assays; With cell suspension finally subcutaneously vaccinated mice and observe silence before and after the AMF genetic liver cell into a tumor ability. Results: The protein expression of AMF in MHCC97-H quantity was higher; Putting the small double-stranded SiRNA transfection into MHCC97-H, the protein expression of AMF significantly reduced (P<0.05); After silence, AMF genetic sequences, MHCC97-H prolifer- ation, migration and invasion forces were significantly decreased (P<0.05); Using cell suspension subcutaneous inoculation of AMF gene in mice MHCC97-H formation of tumor size was smaller than the control group (P<0.05). Conclusion: SiRNA-mediated silencing of AMF gene could significantly inhibit the cell invasion and metastasis of human hepatocellular carcinoma. |
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