贺 帅,吴彦萍,蔡湘仪,陈红波,王丽君,黄来强.姜黄素对宫颈癌细胞生长与增殖的影响研究[J].现代生物医学进展英文版,2017,17(1):7-10. |
姜黄素对宫颈癌细胞生长与增殖的影响研究 |
Study on the Effect of Curcumin on Growth and Proliferation of HeLa Cell |
Received:April 30, 2016 Revised:May 24, 2016 |
DOI:10.13241/j.cnki.pmb.2017.01.002 |
中文关键词: 姜黄素 上游结合因子 核仁转录 聚合酶链式反应 |
英文关键词: Curcumin Upstream binding factor (UBF) Nucleolus transcription Q-PCR |
基金项目:深圳市重点实验室计划以及深圳市科技项目(JCYJ20130402145002438, ZDSYS20140509172959975, GJHZ20140416153844269, JCYJ20140418112611757) |
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中文摘要: |
摘要 目的:探究姜黄素(Curcumin)对宫颈癌细胞HeLa生长与增殖的影响及其作用机制。方法:体外培养HeLa细胞,以0 μmol?L-1、5 μmol?L-1、10 μmol?L-1和20 μmol?L-1等不同浓度的姜黄素处理HeLa细胞;通过MTT和平板克隆实验,研究姜黄素对HeLa细胞生长与增殖的影响;通过免疫荧光共聚焦显微镜技术,研究姜黄素与上游结合因子(Upstream binding factor,UBF)在细胞中的定位情况;通过实时定量聚合酶链式反应(Q-PCR)实验,研究姜黄素对HeLa细胞核仁rDNA转录的影响;利用Western blot技术,研究姜黄素对HeLa细胞中UBF磷酸化的影响。结果:姜黄素对HeLa细胞有明显的生长抑制作用,并呈现出剂量依赖关系;姜黄素降低rDNA的转录水平,下调UBF磷酸化的量,且和UBF在核仁中存在共定位现象。结论:rDNA转录水平的降低和p-UBF的下调可能是姜黄素抑制HeLa细胞生长和增殖的作用途径之一。 |
英文摘要: |
ABSTRACT Objective: To explore the effect of curcumin on growth and proliferation of human cervical cancer HeLa cell, as well as the mechanism. Methods: HeLa cell was treated with different concentrations of curcumin, 0 μmol?L-1, 5 μmol?L-1, 10 μmol?L-1, 20 μmol?L-1, in vitro. MTT and plate cloning experiments were used to study the effect of curcumin on growth of HeLa cell. Immune fluo- rescence confocal experiment was used to study the localization of curcumin and UBF in the cell. The real-time polymerase reaction ex- periment was used to detect the nucleolus transcription of HeLa cell. Western blot was used to study the level of phosphorylation of UBF. Results: Curcumin has an inhibitory effect on the growth of HeLa cell in a dose-dependent manner. Moreover, curcumin is co-localized in the nucleolus with UBF and could down-regulate the level of nucleolus transcription and the level of phosphorylation of UBF. Conclusion: The inhibitory effect may be achieved through the down-regulation of nucleolus transcription and the level of p-UBF. |
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