黄权林 肖冬冬 卢慕峻 李伟 孙康.冷冻干燥及DMEM培养液浸泡对海藻酸水凝胶性能的影响[J].现代生物医学进展英文版,2015,15(35):6816-6821. |
冷冻干燥及DMEM培养液浸泡对海藻酸水凝胶性能的影响 |
The Influence of Lyophilization and Immersion in DMEMon the Propertiesof Alginate Hydrogel |
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DOI: |
中文关键词: 海藻酸水凝胶 力学性能 流变性能 细胞毒性 |
英文关键词: Alginate hydrogel Mechanical property Rheological property Cytotoxicity |
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中文摘要: |
目的:考察冷冻干燥及DMEM培养液浸泡对海藻酸水凝胶性能的影响。方法:制备得到海藻酸水凝胶,对其结构和性能进
行了表征,重点模拟了细胞的培养过程,考察水凝胶- 冻干-DMEM培养液浸泡后水凝胶的力学性能、流变性能的变化。使用
CCK-8 法研究海藻酸水凝胶的细胞毒性。结果:制备了结构和性能可控的海藻酸水凝胶,冻干再浸泡会降低凝胶的拉伸强度和流
变屈服强度。海藻酸水凝胶对上皮细胞和平滑肌细胞没有明显的细胞毒性。结论:冷冻干燥及DMEM培养液浸泡后,凝胶拉伸强
度和屈服强度出现了下降,其原因是浸泡过程中钙离子会从中溶出。 |
英文摘要: |
Objective:To investigate the effect of lyophilization and immersion in DMEM on the properties of alginate hydrogel.Methods:The alginate hydrogel was prepared and the structure and properties of hydrogel were characterized. Above all, the change of
mechanical property and rheological property after lyophilization and immersion in DMEMwere studied by simulating the process of cell
culture. And the cytotoxicity of alginate hydrogel was evaluated with a CCK-8 assay.Results:Alginate hydrogel with controlled structure
and properties were fabricated, the tensile strength and the rheological yield strength of hydrogel decreased after lyophilization and immersion
in DMEM. The alginate hydrogel exhibited no significant cytotoxicity to endothelial cells and smooth muscle cells.Conclusion:After lyophilization and immersion in DMEM, the tensile strength and yield strength of hydrogel decreased, because of the calcium ions
dissolving out of the hydrogel. |
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