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要目的：探讨甘草酸对糖尿病大鼠心肌细胞凋亡和炎症反应的影响。方法：雄性Wistar 大鼠30 只，随机分为3 组：对照（Control）组、糖尿病（DM）组和甘草酸（Gly）组。以高脂高糖饮食及腹腔注射链尿佐菌素30 mg/kg建立糖尿病模型。模型建立后10 周， Gly组每日予甘草酸200 mg/kg 灌胃，DM 和Control组用等量生理盐水灌胃。结果：与Control 组比较，DM组大鼠体重下降，血糖、甘油三酯、总胆固醇及血浆心肌酶水平升高，心肌细胞凋亡增加，心肌Bax、IL-1β、TNF-α、p-NF-κB 、HMGB1 增高而Bcl-2 下降（P<0.05）；与DM 组比较，Gly 组大鼠体重增加，血糖、甘油三酯和总胆固醇及血浆心肌酶水平下降，心肌细胞凋亡减少，心肌 Bax、IL-1β、TNF-α、p-NF-κB 、HMGB1 下降而Bcl-2 升高（P<0.05）。结论：甘草酸治疗可减轻糖尿病大鼠心肌细胞凋亡及炎症反应。

英文摘要:

Objective:To investigate the effects of glycyrrhizin on apoptosis and inflammation in myocardial tissue of diabetic rats.Methods:Thirty male Wistar rats were divided into three groups including the Control group, the Diabetes mellitus (DM) group and the glycyrrhizin (Gly) group randomly. The diabetic model was induced by a dose of streptozoticin (STZ, 30 mg/kg) combined with the high energy diet. Then, the rats in the Gly group received the treatment of 200 mg/kg of glycyrrhizin by garage daily for successively 10 weeks, and the other two groups received the same volume of saline by garage.Results:Compared with Control group, the body weight was lower, and fasting blood glucose, triglyceride and total cholesterol and the serummyocardial enzymes were higher, the ratio of apoptotic cells and the myocardial protein levels of Bax, IL-1β, TNF-α, phospho-NF-κB, HMGB1 were higher in the DM group (P<0.05). Compared with the DM group, the body weight was higher, and fasting blood glucose, triglyceride and total cholesterol and the serum myocardial enzymes were lower, the ratio of apoptotic cells and the myocardial protein levels of Bax, IL-1β, TNF-α, phospho-NF-κB, HMGB1 were lower in the DMgroup (P<0.05).Conclusion:The treatment of glycyrrhizin alleviated apoptosis and inflammation in myocardial tissue of diabetic rats via inhibition of HMGB1 release.

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