| 张红鸭 何方园 田晓甲 史明 贾宏阁.TRAF6 与NF-κB 在特发性炎症性肌病小鼠肌肉中的表达及意义[J].现代生物医学进展英文版,2016,16(32):6231-6234. |
| TRAF6 与NF-κB 在特发性炎症性肌病小鼠肌肉中的表达及意义 |
| The Expression and Significance of TRAF6 and NF-κB in the Muscle Tissueof Idiopathic InflammatoryMyopathies in Mouse |
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| DOI： |
| 中文关键词: 特发性炎症性肌病 肿瘤坏死因子受体相关因子6(TRAF6) 核转录因子κB(NF-κB) |
| 英文关键词: Idiopathic inflammatory myopathies TRAF6 NF-κB |
| 基金项目:国家自然科学基金项目（81171181） |
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| 中文摘要: |
| 目的：研究肿瘤坏死因子受体相关因子6(TRAF6)与核转录因子κB(NF-κB)在特发性炎症性肌病(IIMs)中的表达情况，探讨
TRAF6 在IIMs 发病中的作用及机制。方法：30 只雌性BALB/c 小鼠随机分为5 组(每组6 只)，A：正常对照组；B～E：IIMs 模型自
第一次免疫后分别在1 周、2 周、3 周、4 周末处理组；采用实时荧光定量PCR 方法检测各组小鼠肌肉组织中TRAF6 与NF-κB
mRNA表达水平。结果：(1)IIMs 各组小鼠肌肉中TRAF6 与NF-κB mRNA与正常对照组相比表达均有不同程度升高(P<0.01)，第
2 周末时升高最为显著(P<0.01)，第3 周、4 周呈下降趋势(P<0.01)；(2) IIMs 小鼠各组肌肉组织中TRAF6 与NF-资B mRNA 表达水
平与肌肉炎症程度呈正相关(r=0.940，r=0.908，P<0.01)，前二者之间也呈显著正相关（r=0.944，P<0.01）。结论：TRAF6、NF-κB
mRNA表达在IIMs小鼠肌肉中上调，TRAF6 可能通过NF-资B 的激活在IIMs发生发展过程中发挥重要作用。 |
| 英文摘要: |
| Objective:To investigate tumor necrosis factor receptor associated factor 6 (TRAF6) and nuclear factor kappa B
(NF-κB) mRNAlevels in mouse muscle tissue of idiopathic inflammatory myopathies(IIMs) in order to understand whether TRAF6 plays
a role in IIM.Methods:Thirty female BALB/c mice were randomized for five groups(6 mice in each group): A, normal control mice; B～
E, mice of IIMs model which were sacrificed at the first weekend, the second weekend, the third weekend and the fourth weekend since
they had been given the first immunization for preparing IIMs mouse model. TRAF6 and NF-κB mRNA were detected with real time
fluorescent PCR.Results:(1)Compared with normal control group, the expression of TRAF6 and NF-κB mRNA in each group of IIMs
model had statistically significant differences (P<0.01)and group C had the most (P<0.01).(2)The expression of both TRAF6 mRNA and
NF-κB mRNA had positive correlations with the grading of inflammation respectively (r=0.940, r=0.908, P<0.01). The former two also
had significantly positive correlations (r=0.944, P<0.01).Conclusion:TRAF6 and NF-κBmRNA expressions were upregulated in the
musle tissue of IIMs, which might play an important role in the development of IIMs by activating NF-κB. |
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