| 许栋岳 刘景全 孙雅静 芮岳峰 李克勇.纳米铂黑作为电极镀层的细胞毒性评价[J].现代生物医学进展英文版,2016,16(32):6226-6230. |
| 纳米铂黑作为电极镀层的细胞毒性评价 |
| Evaluation of Cytotoxicity of Nanometer PlatinumBlack as Electrode'sCladding Material |
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| DOI： |
| 中文关键词: 铂黑 细胞毒性 MTS 法 |
| 英文关键词: Platinumblack Cytotoxicity MTS method |
| 基金项目:国家自然科学基金项目(60876082，81070779) |
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| 中文摘要: |
| 目的：探讨将纳米铂黑作为电极镀层的细胞毒性，初步评估铂黑电镀电极长期植入生物体内的安全性。方法：分别改变混悬
液浓度(0.1 mg、0.2 mg、0.3 mg/mL 铂黑混悬液) 对L-929 成纤维细胞培养24 h、48 h、72 h，显微镜下观察细胞形态变化并通过
MTS法计算细胞相对增殖率，对细胞毒性进行评级。结果：0.2 mg/mL、0.3 mg/mL的铂黑混悬液中L-929 成纤维细胞变成圆形、
胞核固缩，细胞稀少，贴壁差；在0.1 mg/mL 的铂黑混悬液中上述细胞形态变化则较轻微。0.1 mg/mL 的铂黑混悬液细胞毒性为
0～2 级，且随时间延长而毒性逐渐减弱并呈现出无细胞毒性；0.2 mg/mL、0.3 mg/mL 的铂黑混悬液细胞毒性为3～4 级，不随时间
变化。结论：纳米铂黑其质量体积浓度低于0.1 mg/mL时具有较好的生物相容性。 |
| 英文摘要: |
| Objective:To evaluate cytotoxicity of nanometer platinum black as an electrode cladding material and to assess
biological security of platinum black electroplating electrode in long-term implantation.Methods:L-929 fibroblasts were cultured with
suspension liquid of the nanometer platinumblack in different concentrations (0.1 mg, 0.2 mg and 0.3 mg/mL). After 24 h, 48 h and72 h,
cells' morphous were observed by microscope, and relative growth rates of cells were calculated via MTS for grading cytotoxicity.Results:L-929 fibroblasts grown into circle, pyknotic nucleus, decreased markedly and mal-adherence in the platinum black suspension
liquid groups (0.2 mg/mL and 0.3 mg/mL), but all above morphology changes of cells were slight in the 0.1 mg/mL platinum black
suspension liquid. The cytotoxicity of platinum black suspension liquid group (0.1 mg/mL) was 0 to 2 degree, with time extended
cytotoxicity weakened gradually and showed no cytotoxicity. However, the cytotoxicity of groups (0.2 mg/mL, 0.3 mg/mL) were 3 to 4
degree and did not change.Conclusion:Nanometer platinum black has good biocompatibility in the mass volume concentration less than
0.1 mg/mL. |
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