| 王泽先 郭菲菲 公衍玲 孙向荣 栾晓 杨丹丹 张孟玲 徐珞.顺铂化疗对下丘脑、血浆ghrelin和orexin 表达的影响[J].现代生物医学进展英文版,2016,16(31):6023-6027. |
| 顺铂化疗对下丘脑、血浆ghrelin和orexin 表达的影响 |
| Effects of Orexin on the Cisplatin-induced Pica in Rats |
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| DOI： |
| 中文关键词: 顺铂 Orexin Ghrelin 大鼠 |
| 英文关键词: Cisplatin Orexin Ghrelin Rat |
| 基金项目:国家自然科学基金项目(81270460，81470815，81500414，81300281)；
山东省优秀中青年科学家科研奖励基金资助项目(BS2014YY009)；青岛市公共领域科技支撑计划(14-2-3-3-nsh) |
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| 中文摘要: |
| 目的：观察顺铂化疗对下丘脑、血浆ghrelin、orexin 表达和摄食量的影响。方法：Real-time PCR、ELISA 法观察顺铂对大鼠下
丘脑、血浆ghrelin、orexin 表达及摄食量的影响；19 名接受顺铂经导管动脉灌注化疗（TAI）的肝细胞患者（HCC），ELISA法检测化
疗前和化疗后血浆ghrelin、orexin 的变化，用直观类比标度（VAS）（0-10）评估食欲和摄食量。结果：每日腹腔注射顺铂6 mg/kg，
1-5 d大鼠摄食量均显著减少（P<0.05），且1-4 d血浆酰化ghrelin 显著降低（P<0.05），5d 时浓度仍低于对照组，但无统计学意义。
血浆非酰化ghrelin 和总的血浆ghrelin 没有明显变化（P>0.05），而1-5 天血浆orexin 水平均明显降低（P<0.05）；顺铂注射1 d 后，
大鼠下丘脑ghreilin 和orexin 的mRNA表达量均显著减少（P<0.05），ghrelin mRNA 变化持续3 d，orexin mRNA 在化疗后5 d仍
低于对照组（P<0.05）；肝细胞癌患者化疗后1 至8 d的摄食量明显降低，1 d和2 d时的血浆酰化ghrelin 显著低于化疗前水平
（P<0.05）。3 d时逐渐恢复，化疗后3 d、4 d和7 d时血浆酰化ghrelin 浓度与化疗前无统计学差异（P>0.05）。血浆非酰化ghrelin 和
总的血浆ghrelin 没有明显变化（P>0.05）；化疗后1～4 d时血浆orexin 浓度均显著降低（P<0.05），化疗后7 d时orexin 基本恢复
到化疗前水平（P>0.05）。结论：顺铂可降低大鼠下丘脑和血浆ghrelin、orexin的mRNA表达，HCC的TAI会降低血浆酰化ghrelin、
orexin、和摄食量。 |
| 英文摘要: |
| Objective:To observe the effect of cisplatin on plasma ghrelin, orexin expression in the hypothalamus and the influence
of food intake.Methods:Real - time PCR and ELISA method to observe the cisplatin on rat hypothalamus, plasma ghrelin, orexin
expression and the influence of food intake; 19 cisplatin transcatheter arterial infusion chemotherapy (TAI) of liver cells (HCC) patients,
ELISA method to detect plasma ghrelin chemotherapy before and after chemotherapy, the change of orexin, using visual analog scale
(VAS) (0 to 10) evaluation appetite and food intake.Results:Daily intraperitoneal injection of cisplatin 6 mg/kg, 1-5 d food intake in rats
were significantly reduced (P<0.05), and 1-4 d plasma acylation ghrelin significantly reduced (P<0.05), 5 d concentration is still lower
than the control group, but no statistical significance. Plasma ghrelin plasma of acylation ghrelin and general no significant change
(P>0.05), and 1-5 days plasma orexin levels were significantly lower (P<0.05); Cisplatin injection after 1 d, rat hypothalamus ghreilin and
orexin mRNA expression were significantly reduced (P<0.05), ghrelin mRNA for 3 d change, orexin mRNA in 5 d after chemotherapy is
still lower than the control group (P<0.05); Hepatocellular carcinoma (HCC) patients after chemotherapy significantly lower intake for 1
to 8 d, 1 d and 2 d plasma acylation ghrelin is significantly below the level before chemotherapy (P<0.05). Returning when 3 d, 3 d, 4 d
and 7 d after chemotherapy when the plasma concentration of acylation ghrelin and chemotherapy before no statistical difference (P>0.05).
Plasma of acylation ghrelin and total plasma ghrelin there was no significant change (P>0.05); After chemotherapy when 1~4 d plasma
orexin concentrations were significantly lower(P<0.05), 7 d after chemotherapy when orexin basic to return to the front of the chemotherapy
(P>0.05).Conclusion:Cisplatin can reduce rat hypothalamus and plasma ghrelin and orexin mRNA expression, HCC TAI lowers
plasma acylation ghrelin, orexin, and food intake. |
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